Difference between revisions of "Team:UNITN-Trento/Test"

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<p>We used different techniques for measuring protein production, such as plate reader, spectrofluorimeter, FACS and for measuring transcript levels by using RT-qPCR</p>
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Revision as of 10:14, 22 August 2015

Interlab Study

I have no idea what I'm doing!

  • Introduction & Achievements

  • Experimental Design

  • Experiments& Protocols

  • Final Discussion & Results

The Interlab Measurement Study

The characterization of standard parts has always been one of the main concerns in Synthetic Biology. For this very same reason, iGEM teams from all around the World were suggested to take part in the biggest Measurement Interlab Study ever conducted and the 2015 UNITN iGEM Team answered the call. The goal of this Second International Measurement Interlab Study is to assemble three different devices, each one containing a promoter with a screening plasmid intermediate and collect as many fluorescence data as possible. The three different promoters will differently affect the GFP production and thating plasmid intermediate and collect as iGEM teams are free to use any technique to measure their devices as long the obtained data are solid and reproducible.

Clear Data and Protocols

We have listed in the ”InterLab Study” page:

  • All devices measured for this study
  • All protocols developed and adopted
  • Sequencing data for all measurements devices

3 BioBrick devices

We used the three BioBrick devices listed in the ”Required Devices” section.

5 techniques

We used in-vivo and in-vitro techniques for measuring RNA and proteins levels:

  • Plate Reader
  • Spectrofluorimeter
  • FACS
  • RT-qPCR
  • Cell-Free Extract

Statistical Reliability

We have three biological replicates for each measurement, with positive and negative controls

Worksheet and Protocol

We have completed the InterLab Worksheet and the InterLab Protocol

Technical replicates

We have technical replicates for each sample

About Safety

We, the UNITN iGEM Team want to follow high standards of safe and responsible biological engineering. Before entering the lab we attended mandatory on-line courses regarding lab safety with final tests. In particular we focused on the following topics:

  • General considerations on risks and prevention
  • Electrical Hazard
  • Chemical Hazard
  • Biological Hazard
  • Compressed gasses and cryogenic liquids

We also attended the “Health and safety at the workplace” course, which is mandatory for all students and workers at the University of Trento.