Difference between revisions of "Team:ETH Zurich/Part Collection"

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<h2> Part Collection</h2>
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<h1> Part Collection</h1>
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<h2>Overview</h2>
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<p>Our whole cancer sensor depends on the sensitivity of the promoter regulated by LldR. LldR is a repressor that binds to O1 and O2 and forms a loop, avoiding any further transcription. If the promoter is too strong it will supress the transcription even in the presene of lactate, which will result in a reduced sensitivity of our system. If the promoter is too weak, it can result in an increased number of false positives. To decide the optimal arrangement of our regulatory system, we designed n promoters by rearranging O1, O2 and a promoter.</p>
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<table>
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<tr>
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<td><h2>Regulatory system design</h2></td>
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<td><h2>BioBrick</h2></td>
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<td>Comments</td>
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</tr>
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<tr>
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<td>lldRO1-plldR-lldRO2</td>
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<td>BBa_K822000</td>
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</tr>
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<tr>
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<td>J23100-lldRO1-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>J23100-lldRO1-R2oDNA-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>lldRO1-J23100-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>J23117-lldRO1-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td><J23117-lldRO1-R2oDNA-lldRO2/td>
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<td></td>
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</tr>
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<tr>
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<td>lldRO1-J23117-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>J23118-lldRO1-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>J23118-lldRO1-R2oDNA-lldRO2</td>
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<td></td>
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</tr>
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<tr>
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<td>lldRO1-J23118-lldRO2</td>
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<td></td>
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</tr>
  
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</table>
  
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<div class="highlightBox">
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<h4>Note</h4>
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<p>In order to be considered for the <a href="https://2015.igem.org/Judging/Awards#SpecialPrizes">Best Part Collection award</a>, you must fill out this page.</p>
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</div>
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<h2>Design of the parts</h2>
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<p>Did your team make a lot of great parts? Is there a theme that ties all your parts together? Do you have more than 10 parts in this collection? Did you make a CRISPR collection, a MoClo collection, or a collection of awesome pigment parts? Describe your parts collection on this page, so the judges can evaluate you for the Best Part Collection award.</p>
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<h2>Chatacterization</h2>
  
  

Revision as of 18:11, 25 August 2015

"What I cannot create I do not understand."
- Richard Feynmann

Part Collection

Overview

Our whole cancer sensor depends on the sensitivity of the promoter regulated by LldR. LldR is a repressor that binds to O1 and O2 and forms a loop, avoiding any further transcription. If the promoter is too strong it will supress the transcription even in the presene of lactate, which will result in a reduced sensitivity of our system. If the promoter is too weak, it can result in an increased number of false positives. To decide the optimal arrangement of our regulatory system, we designed n promoters by rearranging O1, O2 and a promoter.

Regulatory system design

BioBrick

 Comments
lldRO1-plldR-lldRO2 BBa_K822000
J23100-lldRO1-lldRO2
J23100-lldRO1-R2oDNA-lldRO2
lldRO1-J23100-lldRO2
J23117-lldRO1-lldRO2
lldRO1-J23117-lldRO2
J23118-lldRO1-lldRO2
J23118-lldRO1-R2oDNA-lldRO2
lldRO1-J23118-lldRO2

Design of the parts

Chatacterization