Difference between revisions of "Team:Gifu/InterLab"

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<li> <font size="5" face="Arimo"> BioSHAKER TA-12R<br></font> </li>
 
<li> <font size="5" face="Arimo"> BioSHAKER TA-12R<br></font> </li>
 
<font size="4" face="Arimo">
 
<font size="4" face="Arimo">
−
use for shaking culture
+
use for shaking culture</font>
 
  <br><br><br><br>
 
  <br><br><br><br>
 
<li> <font size="5" face="Arimo"> Safire ART-NR:F129013<br></font> </li>
 
<li> <font size="5" face="Arimo"> Safire ART-NR:F129013<br></font> </li>
 
<font size="4" face="Arimo">
 
<font size="4" face="Arimo">
−
use for measurement of absorbance
+
use for measurement of absorbance</font>
 
  <br><br><br><br><br>
 
  <br><br><br><br><br>
 
</ul>
 
</ul>
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&nbsp;&nbsp;We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
 
&nbsp;&nbsp;We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to <i>E. coli</i> K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
 
<br>
 
<br>
−
&nbsp;&nbsp;Setting of plate reader is following:<br>
+
&nbsp;&nbsp;Setting of plate reader is following:<br></font>
 
<img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">
 
<img src="https://static.igem.org/mediawiki/2015/2/20/Set_safari_gify.png" border="0" width="395" height="220">
 
<br><br><br><br>
 
<br><br><br><br>
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<li> <font size="5" face="Arimo"> Definition<br></font> </li>
 
<li> <font size="5" face="Arimo"> Definition<br></font> </li>
 
<font size="4" face="Arimo">
 
<font size="4" face="Arimo">
−
We expressed absorbance as relative value for　positive control. This expression is following:<br>
+
We expressed absorbance as relative value for　positive control. This expression is following:<br></font>
 
<img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"> <br><br><br>
 
<img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"> <br><br><br>
  

Revision as of 04:52, 29 August 2015


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Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement
      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
      Setting of plate reader is following:



Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control. This expression is following:


  • Value
    • Relative absorbance values for positive control are shown below.











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