Difference between revisions of "Team:Gifu/InterLab"

Line 100: Line 100:
 
<li> <font size="5" face="Arimo"> Definition<br></font> </li>
 
<li> <font size="5" face="Arimo"> Definition<br></font> </li>
 
<font size="4" face="Arimo">
 
<font size="4" face="Arimo">
−
We expressed absorbance as relative value for　positive control. This expression is following:<br></font>
+
We expressed absorbance as relative value for　positive control. <br>This expression is following:<br></font>
 
<img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"><br>
 
<img src="https://static.igem.org/mediawiki/2015/8/85/Siki-gifu.png" border="0" width="211" height="57"><br>
 
&nbsp;&nbsp;Sample : Absorbance in sample<br>
 
&nbsp;&nbsp;Sample : Absorbance in sample<br>
Line 111: Line 111:
 
Relative absorbance values for positive control are shown below. <br>
 
Relative absorbance values for positive control are shown below. <br>
 
<img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
 
<img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
 +
  
 
</ul>
 
</ul>
Line 128: Line 129:
 
<ol id="index">
 
<ol id="index">
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
 
<li><a href="https://2015.igem.org/Team:Gifu/InterLab" style="text-decoration:none;" style="text-decoration:none;" >InterLab Study&nbsp;&nbsp;&nbsp;</a></li>
−
<li><a href="https://2015.igem.org/Team:Gifu/Safety"style="text-decoration:none;" >SAFUTY &nbsp; </a></li>
+
<li><a href="#"style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>
 
<li><a href="#" style="text-decoration:none;" > &nbsp; </a></li>

Revision as of 07:07, 29 August 2015


https://static.igem.org/mediawiki/2015/d/d8/Logo_HOME.png https://static.igem.org/mediawiki/2015/a/a4/Logo_PROJECT.png https://static.igem.org/mediawiki/2015/archive/b/b9/20150720104417%21Logo_TEAM.png https://static.igem.org/mediawiki/2015/8/86/Logo_NOTE.png https://static.igem.org/mediawiki/2015/8/87/Logo_RESULT.png https://static.igem.org/mediawiki/2015/2/28/Logo_MORE.png


Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement
      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
      Setting of plate reader is following:



Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control.
    This expression is following:
      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value
    • Relative absorbance values for positive control are shown below.











  MENU
  1. InterLab Study   
  2.  
  3.  
  4.  
  5.  
  6.  
  7.  
  8.  
  9.