Difference between revisions of "Team:Gifu/InterLab"

Line 111: Line 111:
 
Relative absorbance values for positive control are shown below. <br>
 
Relative absorbance values for positive control are shown below. <br>
 
<img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
 
<img src="https://static.igem.org/mediawiki/2015/e/e4/GURAHU-gifu.png" border="0" width="572" height="364"> <br><br>
−
 
+
20A : Device1 (J23101 + I13504)<br>
−
 
+
22A : Device2 (J23106 + I13504)<br>
 +
22K : Device3 (J23117 + I13504)<br><br>
 
</ul>
 
</ul>
 
  <br><br>
 
  <br><br>

Revision as of 07:35, 30 August 2015


https://static.igem.org/mediawiki/2015/d/d8/Logo_HOME.png https://static.igem.org/mediawiki/2015/a/a4/Logo_PROJECT.png https://static.igem.org/mediawiki/2015/archive/b/b9/20150720104417%21Logo_TEAM.png https://static.igem.org/mediawiki/2015/8/86/Logo_NOTE.png https://static.igem.org/mediawiki/2015/8/87/Logo_RESULT.png https://static.igem.org/mediawiki/2015/2/28/Logo_MORE.png


Inter Lab Study


Equipment

  • BioSHAKER TA-12R
    • use for shaking culture



  • Safire ART-NR:F129013
    • use for measurement of absorbance




Method of Measurement

      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were　transferred to 96-well plate by 100mL. We read the absorbance.
      Setting of plate reader is following:



Result and discussion

  • Definition
    • We expressed absorbance as relative value for　positive control.
    This expression is following:
      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value
    • Relative absorbance values for positive control are shown below.


    20A : Device1 (J23101 + I13504)
    22A : Device2 (J23106 + I13504)
    22K : Device3 (J23117 + I13504)




  MENU
  1. InterLab Study   
  2. SAFETY 
  3.  
  4.  
  5.  
  6.  
  7.  
  8.  
  9.