Difference between revisions of "Team:Gifu/InterLab"

Line 121: Line 121:
 
<ul>
 
<ul>
 
<font size="4" face="Arimo">
 
<font size="4" face="Arimo">
&nbsp;&nbsp;The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.
+
&nbsp;&nbsp;The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.<br><br>
 
+
<img src="https://static.igem.org/mediawiki/2015/3/36/Konnsennsasu.png" border="0" width="413" height="105"> <br><br>
  
 
  <br><br><br>
 
  <br><br><br>

Revision as of 01:20, 31 August 2015


https://static.igem.org/mediawiki/2015/d/d8/Logo_HOME.png https://static.igem.org/mediawiki/2015/a/a4/Logo_PROJECT.png https://static.igem.org/mediawiki/2015/archive/b/b9/20150720104417%21Logo_TEAM.png https://static.igem.org/mediawiki/2015/8/86/Logo_NOTE.png https://static.igem.org/mediawiki/2015/8/87/Logo_RESULT.png https://static.igem.org/mediawiki/2015/2/28/Logo_MORE.png



Inter Lab Study


Equipment

  • BioSHAKER TA-12R
  • use for shaking culture



  • Safire ART-NR:F129013
  • use for measurement of absorbance




Method of Measurement

      We constructed 5 plasmids [positive control, negative control, device1~3] by using BioBricks and then transformed to E. coli K-12 JM109. We checked them by colony direct PCR and plasmids sequence. We cultivated correct bacterias by shaking until OD660:0.5. Cultivated mediums were transferred to 96-well plate by 100mL. We read the absorbance.
      Setting of plate reader is following:




Result

  • Definition
  • We expressed absorbance as relative value for positive control.
    This expression is following:

      Sample : Absorbance in sample
      NCav : Average of absorbance in negative control
      PCav : Average of absorbance in positive control



  • Value
  • Relative absorbance values for positive control are shown below.


    FIG1 Relative absorbance values
    20A : Device1 (J23101 + I13504)
    22A : Device2 (J23106 + I13504)
    22K : Device3 (J23117 + I13504)




Discussion

      The value of device1 is much higher than that of device2 and 3. This is why each device is different from promoter sequence, especially -10 and -35 region.