Difference between revisions of "Team:Nagahama/Experiments"
(→Transformation) |
(→LB medium (100 mL liquid)) |
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5.autoclave(121℃ 20min) | 5.autoclave(121℃ 20min) | ||
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| + | <br> | ||
| + | === 2×YT medium (100mL liquid)=== | ||
| + | |||
| + | 1.Measure 1.6g Tripton | ||
| + | |||
| + | 2.Measure 1g Yeast Extract | ||
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| + | 3.Measure 0.5g Nacl | ||
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| + | 4.Add 100mL H2O | ||
| + | |||
| + | 5.autoclave(121℃ 20min) | ||
| + | |||
=== Genome extraction === | === Genome extraction === | ||
↓Cultivate ''E. coli DH5α''using LB media 2ml O/N | ↓Cultivate ''E. coli DH5α''using LB media 2ml O/N | ||
Revision as of 06:37, 31 August 2015
Contents
Protocols
Our Lab's Protocols
Agarose gel(100mL)
Method of Making 0.7% Agarose gel
1.Measure 0.7g Agarose
2.Add 100mL TAE buffer
3.Heat(till agarose melted)*We used a microwave oven.
4.Pur agarose into a gel maker
5.Set a comb
6.Wait till agarose curdles
7.Pull an comb
LB medium (100 mL liquid)
1.Measure 1g Tripton
2.Measure 0.5g Yeast Extract
3.Measure 1g Nacl
4.Add 100mL H2O
5.autoclave(121℃ 20min)
2×YT medium (100mL liquid)
1.Measure 1.6g Tripton
2.Measure 1g Yeast Extract
3.Measure 0.5g Nacl
4.Add 100mL H2O
5.autoclave(121℃ 20min)
Genome extraction
↓Cultivate E. coli DH5αusing LB media 2ml O/N ↓Ultracentrifuge culture 1.5ml (13,000rpm 4℃ 5min) ↓Remove the culture ↓↓↓↓↓↓↓
Plasmids extraction
Transformation by heat shock
Experiments
Experiments & Protocols
Describe the experiments, research and protocols you used in your iGEM project.
What should this page contain?
- Protocols
- Experiments
- Documentation of the development of your project