Difference between revisions of "Team:York/Protocols"

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                 <a href="#LB">Growth Media</a>
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                 <a href="https://2015.igem.org/Team:York/Protocols/Growth_Media">Growth Media</a>
 
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        <h2 style="font-family:Garamond">Lysogeny Broth</h2>
 
        <h3 style="font-family:Garamond">Materials</h3>
 
        <ul>
 
            <li>10g of tryptone</li>
 
            <li>5g of yeast extract</li>
 
            <li>10g of NaCl</li>
 
            <li>1L of Deionized Water</li>
 
            <li> 1M NaCl </li>
 
            <li> 1M KOH </li>
 
        </ul>
 
        <h3 style="font-family:Garamond">Procedure</h3>
 
        <ol>
 
            <li>Use a container with at least double the volume of the liquid that you are making.</li>
 
            <li>Dissolve 10 g tryptone, 5 g yeast extract, and 10 g NaCl in 950 mL deionized water. </li>
 
            <li>Adjust the pH of the medium to 7.0 using 1M NaOH or KOH and bring volume up to 1 liter. </li>
 
            <li>Autoclave. </li>
 
            <li>Store at room temperature or +4°C.</li>
 
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Revision as of 15:02, 31 August 2015

Protocols