Difference between revisions of "Team:UNIK Copenhagen/Construct"

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}
 
}
  
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table.sequence_description {
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background-color: white;
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border-collapse:collapse;
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border:5px solid #0B3B17;
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border-radius:8px; 
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box-shadow: 0px 0px 10px #888888;
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-webkit-box-shadow: 0px 0px 12px #888888;
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-moz-box-shadow: 0px 0px 12px #888888;
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}
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table.sequence_description p {
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font-family: "Lucida sans unicode", sans-serif;
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font-size: 12px;
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margin-left: 10px;
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margin-right: 10px;
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}
  
 
</style>
 
</style>
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<h3>Constructs</h3>
 
<h3>Constructs</h3>
  
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<img src="https://static.igem.org/mediawiki/2015/9/94/UNIK_Copenhagen_Construct_assembly.png" width=80% style="margin:0px 0px 0px 90px">
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<img src="https://static.igem.org/mediawiki/2015/9/94/UNIK_Copenhagen_Construct_assembly.png" width=70% style="margin:0px 0px 0px 100px">
  
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<img src="https://static.igem.org/mediawiki/2015/f/fd/UNIK_Copenhagen_Arrow.png" width=60px style="margin:0px 0px 0px 400px">
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<img src="https://static.igem.org/mediawiki/2015/f/fd/UNIK_Copenhagen_Arrow.png" width=50px style="margin:0px 0px 0px 400px">
  
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<img src="https://static.igem.org/mediawiki/2015/1/1c/UNIK_Copenhagen_Construct.png" width=80% style="margin:0px 0px 0px 90px">
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<img src="https://static.igem.org/mediawiki/2015/1/1c/UNIK_Copenhagen_Construct.png" width=70% style="margin:0px 0px 0px 100px" usemap="#MapGENE" border="0">
  
  
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<map name="MapGENE">
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  <area shape="rect" coords="1,177,156,232" title="Flanking site" type="button" onclick="flankFunction();">
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  <area shape="rect" coords="157,177,230,232" title="Kanamycin casette" type="button" onclick="nptIIFunction();">
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  <area shape="rect" coords="227,177,252,232" title="ZmUbi promoter" type="button" onclick="ZmUbiFunction();">
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  <area shape="rect" coords="348,177,430,232" title="Gene of interest" type="button" onclick="GOIFunction();">
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  <area shape="rect" coords="431,177,441,232" title="Linker" type="button" onclick="LinkerFunction();">
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  <area shape="rect" coords="439,177,610,232" title="YFP" type="button" onclick="YFPFunction();">
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  <area shape="rect" coords="609,177,644,232" title="Terminator" type="button" onclick="TerminatorFunction();">
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  <area shape="rect" coords="642,177,806,232" title="Flanking site" type="button" onclick="flankFunction();">
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</map>
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<table class="sequence_description" id="about_gene"></table></div>
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<script>
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function flankFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About the flanking sites...</p>";
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}
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function nptIIFunction() {
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    document.getElementById("about_gene").innerHTML="<p>Gene that encodes for kanamycin resistance for selection of transformants.</p>";
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}
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function ZmUbiFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About the promoter...</p>";
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}
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function GOIFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About antifreeze gene and STS gene...</p>";
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}
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function LinkerFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About the liinker...</p>";
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}
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function YFPFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About YFP...</p>";
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}
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function TerminatorFunction() {
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    document.getElementById("about_gene").innerHTML="<p>About the terminator...</p>";
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}
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</script>
  
  
 
</html>
 
</html>

Revision as of 13:26, 1 September 2015


Under construction...

Anti-freeze protein

Resveratrol

Resveratrol is an antioxidant. Resveratrol is synthesized from p-Coumaroyl-CoA by stilbene synthase (STS) making the final enzyme in the resveratrol biosynthesis. Moss already produces the precursor p-Coumaroyl-CoA, which makes it simple to engineer moss to produce resveratrol.

The construct for introduction and expression of STS contains are flanked by regions homologous to the moss genome so that the STS-gene will integrate by homologous recombination in moss. The constructs furthermore contains the ZmUbi-promoter, a kanamycin resistance gene and YFP, to confirm transformation.

Constructs