Difference between revisions of "Team:UNIK Copenhagen/Construct"

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   <area shape="rect" coords="1,130,120,190" title="Flanking site" type="button" onclick="flankFunction();">
 
   <area shape="rect" coords="1,130,120,190" title="Flanking site" type="button" onclick="flankFunction();">
 
   <area shape="rect" coords="121,130,178,190" title="Kanamycin casette" type="button" onclick="nptIIFunction();">
 
   <area shape="rect" coords="121,130,178,190" title="Kanamycin casette" type="button" onclick="nptIIFunction();">
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   <area shape="rect" coords="179,130,280,190" title="ZmUbi promoter" type="button" onclick="ZmUbiFunction();">
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   <area shape="rect" coords="179,130,276,190" title="ZmUbi promoter" type="button" onclick="ZmUbiFunction();">
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   <area shape="rect" coords="281,130,310,190" title="Gene of interest" type="button" onclick="GOIFunction();">
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   <area shape="rect" coords="277,130,330,190" title="Gene of interest" type="button" onclick="GOIFunction();">
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   <area shape="rect" coords="311,130,314,190" title="Linker" type="button" onclick="LinkerFunction();">
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   <area shape="rect" coords="331,130,350,190" title="Linker" type="button" onclick="LinkerFunction();">
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   <area shape="rect" coords="315,130,427,190" title="YFP" type="button" onclick="YFPFunction();">
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   <area shape="rect" coords="351,130,427,190" title="YFP" type="button" onclick="YFPFunction();">
 
   <area shape="rect" coords="426,130,448,190" title="Terminator" type="button" onclick="TerminatorFunction();">
 
   <area shape="rect" coords="426,130,448,190" title="Terminator" type="button" onclick="TerminatorFunction();">
 
   <area shape="rect" coords="449,130,600,190" title="Flanking site" type="button" onclick="flankFunction();">
 
   <area shape="rect" coords="449,130,600,190" title="Flanking site" type="button" onclick="flankFunction();">

Revision as of 13:42, 1 September 2015


Under construction...

Anti-freeze protein

Resveratrol

Resveratrol is an antioxidant. Resveratrol is synthesized from p-Coumaroyl-CoA by stilbene synthase (STS) making the final enzyme in the resveratrol biosynthesis. Moss already produces the precursor p-Coumaroyl-CoA, which makes it simple to engineer moss to produce resveratrol.

The construct for introduction and expression of STS contains are flanked by regions homologous to the moss genome so that the STS-gene will integrate by homologous recombination in moss. The constructs furthermore contains the ZmUbi-promoter, a kanamycin resistance gene and YFP, to confirm transformation.

Constructs