Difference between revisions of "Team:Sherbrooke/Practices"
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<p> | <p> | ||
1. OpenPCR cannot withstand a 96-well plate, but is able to cycle 1.5mL tubes.</br> | 1. OpenPCR cannot withstand a 96-well plate, but is able to cycle 1.5mL tubes.</br> | ||
− | 2. ThermosStat plus is designed to accommodate either 24 x 1.5/2.0mL tubes or 8 x 50mL tubes or 1 x 96-well plate | + | 2. ThermosStat plus is designed to accommodate either 24 x 1.5/2.0mL tubes or 8 x 50mL tubes or 1 x 96-well plate. |
</p> | </p> | ||
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<h4>Specifications</h4> | <h4>Specifications</h4> | ||
<p> | <p> | ||
+ | The modules to implement are described as follows: | ||
+ | </p> | ||
+ | <p>The first module, MC96:</p> | ||
+ | <ul> | ||
+ | <li>Suitable for a 96-well plate (common sized plate for biological manipulations).</li> | ||
+ | <li>Control and maintain temperature cycling between 0 to 80°C±1.5°C.</li> | ||
+ | <li>Achieve a cooling and heating ramp of 0.5 to 1°C/s.</li> | ||
+ | <li>Apply an electromagnetic field on demand.</li> | ||
+ | <li>Less than 1000$.</li> | ||
+ | </ul> | ||
+ | </br> | ||
+ | <p>The second module, MC1.5:</p> | ||
+ | <ul> | ||
+ | <li>Suitable for a test tube of 1.5mL. </li> | ||
+ | <li>Capable of independent control for each unit of three tubes.</li> | ||
+ | <li>Control and maintain temperature cycling between 0 to 80°±1.5°C.</li> | ||
+ | <li>Achieve a cooling and heating ramp 0.5 to 1°C/s.</li> | ||
+ | <li>Apply an electromagnetic field on demand.</li> | ||
+ | <li>Less than 1000$.</li> | ||
+ | </ul> | ||
+ | </br> | ||
+ | <p>The third module, TAC:</p> | ||
+ | <ul> | ||
+ | <li>Suitable for a glass tube having a diameter of 25mm, capacity of 50mL. </li> | ||
+ | <li>Independent control for each tube.</li> | ||
+ | <li>Control and maintain temperature cycling between 0 to 37°±1.5°C.</li> | ||
+ | <li>Achieve a heating ramp of 0.08°C/s.</li> | ||
+ | <li>Achieve a cooling ramp of 0.1°C/s above room's temperature.</li> | ||
+ | <li>Achieve a cooling ramp of 0.025°C/s below room's temperature.</li> | ||
+ | <li>Apply a magnetic stirring to the liquid (Mixing of bacterial cultures).</li> | ||
+ | <li>Calculate the optical density of the liquid (Transmittance monitoring).</li> | ||
+ | <li>Less than 1000$.</li> | ||
+ | </ul> | ||
− | + | <p> | |
+ | All of the modules must respect dimensions that are multiples of a 96-well plate. These dimensions are imposed so | ||
+ | that our modules respect the template established by the OpenTrons team for their OT.One robotic platform. It is | ||
+ | also important to note that this format is commonly used in laboratories and is a standard in biology. According | ||
+ | to this, the MC96 module was designed to fit into the size of one plate and it will have everything needed to | ||
+ | control a single 96-well plate. The MC1.5 module will include four different blocks in the space of one 96-well | ||
+ | plate, each containing three 1.5mL tubes with the electronics and the physical supports required for each block. | ||
+ | Finally, the TAC module will include two glass tubes of 25mm and all the electronics and supports required to | ||
+ | control them individually. Each of our modules is then shorter and thinner than the size of a single 96-well plate. | ||
+ | </p> | ||
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Revision as of 18:35, 1 September 2015
Human Practices
Feasability
Platform
State of the art
Figure 1 - «RapidHit» platform from Hudson Robotics
Figure 2 - «epMotion 5075v» from Eppendorf
Figure 3 - OpenTrons OT.One platform
Specifications
Some specifications were taken in consideration during the designing process of the robotic platform. While respecting those specifications, the thought process behind the design kept as main focus to deliver a customizable platform, low cost but still effective. The principal specifications were:
- Movements of the robotic platform must have a 1 mm precision due to the narrowness of the micro plates’ spacing between wells
- Must be able to use different kinds of pipette tools at the same time and change tools with ease.
- Gripper must be precise enough, without putting excessive force, to grab as small as 1.5 mL tubes and as large as 96-well plates. It also needs to have a range of opening from 0 mm to 85 mm
- Centrifuge needs to rotate at a speed capable of exerting a minimum gravitational force of 6000G and must be equipped with security devices such as detection of abnormal vibration or securing the lid after closing
- Complete platform must be fully open-hardware and detailed at no more than 10000$
Specifications achieved
Some specifications have already been achieved (the others will be achieved by December 2015):
- Movements of the robotic platform must have a 1 mm precision
- Complete platform must be fully open-hardware and detailed at no more than 10000$
Approximate cost by parts
Parts | Cost(USD) |
---|---|
Platform frame’s pieces | 600$ |
Motor’s drive | 59$ |
Cable track | 53$ |
X/Y Motors | 65$ |
Gripper | 300$ |
Screw for the x/y axis | 250$ |
Single channel pipette (not include) | 100$ |
Multi-channel pipette (not include) | 300$ | Total | 1727$ |
Modules
State of the art
Actually, there are different robots available on the market that are capable of performing the tasks and the functions that are to be implemented by our modules. However, most of them are only capable of doing one specific task and this, at a significantly higher cost than what is desired for our project. As an example, Table 1 presents different options available on the market to regulate the temperature of a 96-well plate. It is to be noted that these options only control the temperature cycles, which represents only one of the capabilities implemented by our modules.
Table 1: Comparative Table of Temperature Cycling Module
Manufacturer | Product Name | Price(USD) | Temperature range(°C) | Temperature speed(°C/s) |
---|---|---|---|---|
Life Technologies | SimpliAmpTM Thermal Cycler | 4 995,00$ | 0 - 100°C ±0.25°C | 4°C/s |
OpenPCR | Open Source PCR Thermalcycler | 649,00$ | 10 - 105°C ±0.5°C | 1°C/s |
Eppendorf | Mastercycler® nexus | 7 200,00$ | 4-99°C ±0.2°C | 2.5°C/s |
Inheco | On Deck Thermal Cycle-ODTC 96 | On demand | 4-99°C ±0.3°C | 3.3°C/s |
Eppendorf | ThermoStat plus | Discontinued | 1-99°C ±1°C | 1.5°C/s |
1. OpenPCR cannot withstand a 96-well plate, but is able to cycle 1.5mL tubes. 2. ThermosStat plus is designed to accommodate either 24 x 1.5/2.0mL tubes or 8 x 50mL tubes or 1 x 96-well plate.
Specifications
The modules to implement are described as follows:
The first module, MC96:
- Suitable for a 96-well plate (common sized plate for biological manipulations).
- Control and maintain temperature cycling between 0 to 80°C±1.5°C.
- Achieve a cooling and heating ramp of 0.5 to 1°C/s.
- Apply an electromagnetic field on demand.
- Less than 1000$.
The second module, MC1.5:
- Suitable for a test tube of 1.5mL.
- Capable of independent control for each unit of three tubes.
- Control and maintain temperature cycling between 0 to 80°±1.5°C.
- Achieve a cooling and heating ramp 0.5 to 1°C/s.
- Apply an electromagnetic field on demand.
- Less than 1000$.
The third module, TAC:
- Suitable for a glass tube having a diameter of 25mm, capacity of 50mL.
- Independent control for each tube.
- Control and maintain temperature cycling between 0 to 37°±1.5°C.
- Achieve a heating ramp of 0.08°C/s.
- Achieve a cooling ramp of 0.1°C/s above room's temperature.
- Achieve a cooling ramp of 0.025°C/s below room's temperature.
- Apply a magnetic stirring to the liquid (Mixing of bacterial cultures).
- Calculate the optical density of the liquid (Transmittance monitoring).
- Less than 1000$.
All of the modules must respect dimensions that are multiples of a 96-well plate. These dimensions are imposed so that our modules respect the template established by the OpenTrons team for their OT.One robotic platform. It is also important to note that this format is commonly used in laboratories and is a standard in biology. According to this, the MC96 module was designed to fit into the size of one plate and it will have everything needed to control a single 96-well plate. The MC1.5 module will include four different blocks in the space of one 96-well plate, each containing three 1.5mL tubes with the electronics and the physical supports required for each block. Finally, the TAC module will include two glass tubes of 25mm and all the electronics and supports required to control them individually. Each of our modules is then shorter and thinner than the size of a single 96-well plate.