Revision as of 11:21, 31 May 2015

香蔵庫(KOZOKO)

~This is our new HERO!!~

Project

Back Graund

We use erectric power on a massive scale everyday. please look at figure A below.

Plasmid

（The under）Geraniol synthesis circuit. m-ispA which we improved to synyhesize only GPP, and not to synthesize FPP、GES derived from Ocimum Basilicum、we let Escherichia coli overexpress transcription factor MarA letting the expression of pump that the film outside exhausts geraniol activate. (The below）MEP strengthening path way。We can let Escherichia coli make materials GPP of various terpenoids, FPP, GGPP in large quantities by strengthening MEP course.

MEP Pathway

A non-mevalonate (MEP) path way is a biosynthesis path way of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).

It is the slightly complicated synthetic path way with six intermediary metabolite before Escherichia coli synthesizes IPP and DMAPP.

A path way synthesizing same IPP and DMAPP includes a mevalonate path way.

The difference of these path way is two.

The first difference.

A starting material is the acetyl CoA of 3 molecules by the mevalonate path way.

In contrast, a starting material is pyruvic acid and Glyceraldehyde 3-phosphate by the non-mevalonate pathway.

The second difference.

It is a mevalonate pathway to make mevalonic acid a biosynthesis intermediate.

That does not make a biosynthesis intermediate is non-mevalonate pathway.

Many bacteria and chloroplast of plants has the non-mevalonate pathway.

Escherichia coli synthesizes IPP and DMAPP necessary to produce beta-carotene and quinone or terpene by non-mevalonate pathway.

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