Difference between revisions of "Team:UNIK Copenhagen/Results"
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| − | <p style="font-size:10.5px; margin: 4px 0px 0px -117px"> Figure 4: Fluorescence microscopy pictures of P. patens transformed with our genetic constructs. A) A moss protoplast transformed with the antifreeze construct. B) A moss protoplast transformed with our STS construct. C) A moss protoplast transformed with a vector expressing YFP. A positive control. D) WT moss. A negative control. 1) Bright field picture. 2) Filter showing autoflouorescence (red) and YFP-expression (green). 3) Filter | + | <p style="font-size:10.5px; margin: 4px 0px 0px -117px"> Figure 4: Fluorescence microscopy pictures of P. patens transformed with our genetic constructs. A) A moss protoplast transformed with the antifreeze construct. B) A moss protoplast transformed with our STS construct. C) A moss protoplast transformed with a vector expressing YFP. A positive control. D) WT moss. A negative control. 1) Bright field picture. 2) Filter showing autoflouorescence (red) and YFP-expression (green). 3) Filter showing only YFP-expression (green).</p style> |
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Revision as of 13:10, 6 September 2015
Project Results
A few days after transformation we observed the moss protoplasts under a fluorescent microscope and saw YFP expression in moss protoplats for the antifreeze and the STS construct (fig. 4A and 4B). This confirms the that our transformation was a success and highly suggests that our genes of interest, antifreeze and STS, are expressed. It demonstrated that our construct design works and that moss can combine different DNA pieces with matching overhangs using homologous recombination.
Figure 4: Fluorescence microscopy pictures of P. patens transformed with our genetic constructs. A) A moss protoplast transformed with the antifreeze construct. B) A moss protoplast transformed with our STS construct. C) A moss protoplast transformed with a vector expressing YFP. A positive control. D) WT moss. A negative control. 1) Bright field picture. 2) Filter showing autoflouorescence (red) and YFP-expression (green). 3) Filter showing only YFP-expression (green).
Here you can describe the results of your project and your future plans.
What should this page contain?
- Clearly and objectively describe the results of your work.
- Future plans for the project
- Considerations for replicating the experiments
Project Achievements
You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.
- A list of linked bullet points of the successful results during your project
- A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.
Inspiration
See how other teams presented their results.