Difference between revisions of "Team:London Biohackspace/lab-book"

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                   <h2>Protocols</h2>
 
                   <h2>Protocols</h2>
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/liiquid-yepd">Preparing liquid YEPD media</a></h3>
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/liiquid-yepd">Preparing liquid YEPD media</a></h3>
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                      <p>1. For 1 litre of YEPD media measure the following into an erlenmayer or duran flask<br/><br/>
 
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                      Bacto yeast extract (Difco 0127-17) (1%) 10g<br/>
 
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                      Bacto peptone (Difco 0118-17) (2%) 20g<br/>
 
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                      Glucose (2%) 20g<br/><br/>
 
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                      Available premixed as Difco 0428-17.<br/><br/>
 
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                      2.  Autoclave for at least 15 minutes at 121C<br/>
 
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                      </p>
 
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/yepd-agar-plates">Preparing YEPD agar plates</a></h3>
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/yepd-agar-plates">Preparing YEPD agar plates</a></h3>
−
                      <p>1. For 1 litre of YEPD media measure the following into an erlenmayer or duran flask<br/><br/>
 
−
                      Bacto yeast extract (Difco 0127-17) (1%) 10g<br/>
 
−
                      Bacto peptone (Difco 0118-17) (2%) 20g<br/>
 
−
                      Glucose (2%) 20g<br/><br/>
 
−
                      Available premixed as Difco 0428-17.<br/><br/>
 
−
                      Bacto agar (Difco 0140-01) (2%) 20g<br/><br/>
 
−
                      2.  Autoclave for at least 15 minutes at 121C<br/>
 
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                      2.  Pour 20ml into an agar plate.  Leave to cool at room temperature for 2-3 hours and store at -4C<br/>
 
−
                      </p>
 
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/ecoli-tansformation">Electroporation Transformation of <i>E. coli</i> DH5aplha</a></h3>
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/ecoli-tansformation">Electroporation Transformation of <i>E. coli</i> DH5aplha</a></h3>
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                      <p><ul>
 
−
                          <li><a href="#">Protocol</a></li>
 
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                      </ul></p>
 
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/yeast-tansformation">Electroporation Transformation of <i>S. cerevisiae</i></a></h3>
 
                       <h3><a href="https://2015.igem.org/Team:London_Biohackspace/protocols/yeast-tansformation">Electroporation Transformation of <i>S. cerevisiae</i></a></h3>
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                      <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p>
 
  
 
                   <h2>Experiments</h2>
 
                   <h2>Experiments</h2>

Revision as of 14:24, 6 September 2015

BEER.CO - Royal quality

  • Lab Book

Protocols

Preparing liquid YEPD media

Preparing YEPD agar plates

Electroporation Transformation of E. coli DH5aplha

Electroporation Transformation of S. cerevisiae

Experiments

Expressing Miraculin in S. cerevisiae

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Expressing Lycopene in S. cerevisiae

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Creating Leucine and Uracil auxotrophic S. cerevisiae strains

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RNAi based regulation of mRFP in E. coli

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Results

Expressing Miraculin in S. cerevisiae

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Expressing Lycopene in S. cerevisiae

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Creating Leucine and Uracil auxotrophic S. cerevisiae strains

Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.

RNAi based regulation of mRFP in E. coli

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