Difference between revisions of "Team:Dundee/CGCraigon"
Line 136: | Line 136: | ||
<p><b>Next Steps:</b> The next step will be to run the PCR product on a gel and perform a gel extraction on the part of the gel corresponding to the size of the <I>OBP2A</I> gene fragment. </p> | <p><b>Next Steps:</b> The next step will be to run the PCR product on a gel and perform a gel extraction on the part of the gel corresponding to the size of the <I>OBP2A</I> gene fragment. </p> | ||
</div> | </div> | ||
+ | |||
+ | |||
+ | </div> | ||
<span class="box-title">23/6: Gel extraction of <I>OBP2A</I> from the PCR performed on the 22/6 </span> | <span class="box-title">23/6: Gel extraction of <I>OBP2A</I> from the PCR performed on the 22/6 </span> | ||
<span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#nmcollapsiblew2-2></span> | <span type="button" class="chevron_toggleable box-btn glyphicon glyphicon-chevron-down" data-toggle="collapse" data-target="#nmcollapsiblew2-2></span> | ||
Line 144: | Line 147: | ||
<p><b>Next Steps:</b> The next step will be to run the PCR product on a gel and perform a gel extraction on the part of the gel corresponding to the size of the <I>OBP2A</I> gene fragment. </p> | <p><b>Next Steps:</b> The next step will be to run the PCR product on a gel and perform a gel extraction on the part of the gel corresponding to the size of the <I>OBP2A</I> gene fragment. </p> | ||
</div> | </div> | ||
− | |||
− | |||
</div> | </div> | ||
</div> | </div> |
Revision as of 21:57, 6 September 2015
Watch Our Introduction Video
Summary
Produced a plasmid preperation of pIDT-OBP2A in order to use it as template in PCR.
Aim of Experiment: To transform pIDT-OBP2A into MC1061 e.coli strain
Protocols Used: Transformation
Results: N/A
Next Steps: If the transformation is successful the next step will be to produce an overnight culture for plasmid preparation.
Aim of experiment: To produce an overnight culture of pIDT-OBP2A from positive colonies of MC1061 E.coli strain from the transformation done on the 17/6.
Protocols Used: Overnight Cultures
Results: N/A
Next Steps: Produce a plasmid preparation of pIDT-OBP2A from this overnight culture.
Aim of experiment: The overnight culture produced yesterday will now undergo a plasmid preparation in order to obtain the pIDT-OBP2A plasmid.
Protocols Used: Miniprep
Results: The plasmid preperation yeilded a concentration of 305.53 ng/ul.
Next Steps: To run a PCR using the purified pIDT-OBP2A plasmid as a template for amplification.
Summary
Removal of the signaling peptide from OBP2A and insertion of OBP2A into the biobrick vector pSB1C3.
Aim of Experiment: To perform a PCR on the plasmid preparation of pIDT-OBP2A produced last week, using primers that have been designed in a way as to remove the signalling peptide at the start of the OBP2A gene.
Protocols Used: PCR
Results: N/A
Next Steps: The next step will be to run the PCR product on a gel and perform a gel extraction on the part of the gel corresponding to the size of the OBP2A gene fragment.