Difference between revisions of "Team:UCL/Practices"

How?

We've been involved in discussions with patients, practitioners,scientists and volunteers in charities to inquire about mental health issues. We also plan to use this as an opportunity to create awareness for the gut-brain axis which is quite a nascent field of research. This helps us ensure the relevance of our project to patients.

We've organized events in collaboration with artists, museums and mental health organisations in order to start discussions with the general public and incorporate their ideas into our project. As of now there are two aspects that are needed to be considered:

• Firstly, a large part of scientific research is not accessible to the general public due to technical language and jargon. This barrier needs to be overcome in order to inform the public about the physiology of mental health and to help the patients understand their condition.


• Secondly, patients taking medication (e.g. for depression) must increase their dose in controlled, regular intervals. This is an issue we will consider when building the genetic circuit for our biobricks.

Art and design

• Mood enhancement Collection

  Through our art and design we aimed to analyse and explore the future implications of delivering genetically modified bacteria to possibly enhance our bodies and minds. Furthermore, we wanted to bring our project to the general public, by creating a collection of pictures with potential products that could be manufactured to deliver our genetically enhanced bacteria.

  • We collaborated with bioartists Anna Dumitriu and Heather Mac to produce a range of new products for the Hypersymbiont Enhancement Salon collection. Anna Dumitriu is a renowned visual artist whose works blurs the boundaries between art and science with a strong interest in microbiology, synthetic biology and art/science ethics.
  • Anna used our engineered E.coli overexpressing serotonin to develop a fun and creative collection of everyday products containing the psychobiotic that makes people ‘happier’. In order to do so we shipped the probiotic strain E.coli Nissle containing the tryptophan hydroxylase expressing plasmid to The University of Sussex, where Anna based her work.
  • The GM E.coli was then freezed dried in order to sparkle this ‘shiny dust’ on the toothpaste and lipstick, while live bacteria was used for the tabs and jellies. The result has been an amazing collection of products that greatly show the possibilities of synthetic biology, particularly by manufacturing treatments to improve human health.

Ethics

This year, UCL iGEM team has decided to take a lead in debating the implications of our project and engaging with the public to address the ethical concerns raised by synthetic biology. Our team has therefore been looking deeply into the ethics of synthetic biology, particularly when it is applied to human health, as nowadays multiple social and ethical challenges are arising as a result of the development and expansion of this promising field.

• General public survey

  We wanted to know the general public perception on the possibility of manufacturing probiotics containing engineered bacterial strains, and what their view on the ethics of this procedure and synthetic biology in general was. Furthermore, we asked them what they thought the future implications and concerns of synthetic biology could be and whether they believed that it would be beneficial for society or not. In order to do so we prepared a short survey for people to fill in through social media, and then we analysed the results and built the statistics.

  Our survey was translated into 5 different languages including Spanish, French, Portuguese and German as we believed that this would help us reach a broader range of people and therefore provide us with more significant data.

  Knowing the public perception in a novel and sometimes controversial field such as synthetic biology will allow us to tackle some of the ethical questions and concerns raised by society in order to maintain the public legitimacy and support.

• UCL Ethics

  We have been working closely with UCL Ethics to make sure our project met the requirements set by our university regarding the safeguarding of rights, safety, dignity and well-being of research participants. All our interviews and video assays were approved by the UCL Chair of the Departamental Ethics Comitee. Previous to the interview the participants were informed about the nature of the research, the format of the questions they will be asked, their content, their rights, and how we would treat the information they provided us. Moreover, we distributed informed consent forms to all participants to ensure that they provided fully consent before participating in the interviews.

Safety

Besides working on an effective biocontainment mechanism for our genetically modified probiotic strains we wanted to make sure that our team followed all the safety procedures and requirements in order to minimise any potential risks that our bacteria could represent to the environment and human-health.

Our team has strictly followed the safety guidelines set by iGEM as well as UCL Biochemical Engineering department. Before conducting any work in the lab all the team members received a Safety training by the UCL Biochemical Engineering Dept Biosafety Officer Dr Brian O’Sullivan. We have also checked that all the materials, probiotic and cell lines used fell into the iGEM White list as well as elaborate risk assessments for all the bacterial strains and hazardous materials used. We decided to Check in for approval both of the mammalian cell lines we worked with as they were classified as Risk 2 microorganisms. The Cell lines included:

• Caco-2 Cell line from Human epithelial colorectal adenocarcinoma cells
• COS-7 Cell line from African Green Monkey Kidney cells