Difference between revisions of "Team:Gifu/team-profile"

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<a name="instructor" style="text-decoration:none;"><font color="000000"size="6"> TEAM PROFILE </a></font> <br>
 
<a name="instructor" style="text-decoration:none;"><font color="000000"size="6"> TEAM PROFILE </a></font> <br>
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<tbody><tr><td width="150">Team Name: <td style="line-height: 150%; font-size: 150%; font-weight: 800;" colspan="9">Gifu<div style="padding-left: 40px; font-size: 12px; font-weight: normal; display: inline;">
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            <a href="https://2014.igem.org/Team:Gifu">(Visit their wiki)</a><a style="padding-left: 20px;" href="http://partsregistry.org/cgi/dna_transfer/batch_list.cgi?group_id=1707"> (Part Submissions)</a></div><tr><td>Primary Contact (PI): <td colspan="9">Akio Ebihara<tr><td style="vertical-align: top;">Schools:<td colspan="9"><pre style="background: none; padding: 0px; border: currentColor; border-image: none; width: 90%;">Gifu University
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Yanagido, Gifu, Japan</pre><tr><td>Division:<td style="width: 300px;">iGEM<td>Application Date:&nbsp; 2014-02-17<tr><td>Section:<td style="width: 300px;">Undergraduate<tr><td>Region:<td style="width: 300px;">Asia<td>Acceptance Date:  2014-04-23 12:49:42<tr><td>Description:<td colspan="9">
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<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Team Status</b><td align="center"><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
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                <b>Welcome to iGEM 2014 !</b> Your iGEM 2014 team application was accepted by iGEM Headquarters on 2014-04-23 12:49:42
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                and your team registration fee has been received.</tr></tbody></table><div style="height: 3px;"></div><table_break></table_break><table width="800" id="table_status" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
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<tbody><tr><td><img src="https://igem.org/images/green_check.png"> DNA Kit of Parts shipping information has been entered, thanks.
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                <a href="Shipping.cgi?group_id=1707"><i> View shipping information</i></a><tr><td><img src="https://igem.org/images/green_check.png"> Resource description has been submitted, thanks.
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                <a href="Resource_Description.cgi?group_id=1707"><i> View resource description</i></a><tr><td><img src="https://igem.org/images/green_check.png"> Your registration fee has been received. Thank you.
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                <a href="https://2014.igem.org/Team_Registration_Fee"><i> More...</i></a><table_break>
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<tbody><tr><td colspan="9">Assigned Track: New Application
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<table width="800" style="border: currentColor; border-image: none; margin-right: auto; margin-left: auto;" border="0" cellspacing="0" cellpadding="0"><tbody><tr><td width="30%" nowrap="" style="text-align: left; padding-right: 2em;"><b>Title and Abstract</b><a style="color: rgb(102, 102, 255); padding-left: 15px; font-size: 90%;" href="https://2014.igem.org/Jamboree/Title_and_Abstract"><i>More...</i></a><td align="center"><a style="color: blue; text-decoration: none;" href="abstract_pdf_file.cgi?id=1332"><i>Download a .pdf version</i></a><td width="30%" align="right"></tr></tbody></table><table width="800" id="table_abstract" style="padding: 3px 2px 0px 15px; border: 1px solid gray; border-image: none; line-height: 150%; margin-right: auto; margin-left: auto; background-color: rgb(220, 220, 220);" border="0" cellspacing="0" cellpadding="0">
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<tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br>
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<tr><td style="padding: 5px 0px 5px 20px; width: 750px; vertical-align: top;" colspan="9"> Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive　one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming <i>E.coli</i>, we get world’s longest proteins.
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<tbody><tr><td style="padding: 3px 0px;"><b>Instructors</b>
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<!-- Non-editable Member 23221 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=19993">aebihara2014</a><td style="width: 200px;">Akio Ebihara<td><td>
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<!-- Non-editable Member 23933 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20610">isatoshi</a><td style="width: 200px;">Satoshi IWAMOTO<td><td>
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<!-- Non-editable Member 24452 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21012">h1884</a><td style="width: 200px;">Hitoshi IWAHASHI<td><td></tr></tbody></table><div style="height: 3px;"></div><table_break>
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<tbody><tr><td style="padding: 3px 0px;"><b>Student Members</b>
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<!-- Non-editable Member 23894 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20590">ybanno</a><td style="width: 200px;">Yusuke Banno<td><td>
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<!-- Non-editable Member 23895 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20591">nomuken</a><td style="width: 200px;">Nomura Kenta<td><td>
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<!-- Non-editable Member 23896 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20592">momochi</a><td style="width: 200px;">Momoko YAMADA<td><td>
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<!-- Non-editable Member 23897 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20593">kozakai</a><td style="width: 200px;">Kozakai Tomoya<td><td>
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<!-- Non-editable Member 23898 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20594">Mori</a><td style="width: 200px;">Akihiro Moriyama<td><td>
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<!-- Non-editable Member 23899 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20596">Yuki1201</a><td style="width: 200px;">Yuki Kamijo<td><td>
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<!-- Non-editable Member 23900 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20595">SachiA</a><td style="width: 200px;">Sachi Asano<td><td>
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<!-- Non-editable Member 23901 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=20597">takema</a><td style="width: 200px;">Hasegawa Takema<td><td>
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<!-- Non-editable Member 24451 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21011">fukufuku</a><td style="width: 200px;">Wataru Fukuda<td><td>
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<!-- Non-editable Member 24477 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21022">Lagomutry</a><td style="width: 200px;">Ouchi Ryo<td><td>
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<!-- Non-editable Member 24761 --><tr><td style="width: 150px; padding-left: 10px;"><a href="User_Information.cgi?user_id=21240">uyuu</a><td style="width: 200px;">Endo Yuki<td><td>
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<tbody><tr><td>This range of part numbers has been assigned to your team for use during the summer: <span style="margin-left: 10px;">BBa_K1332000 to BBa_K1332999</span>
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Revision as of 00:05, 14 September 2015



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TEAM PROFILE
Team Name: Gifu
Primary Contact (PI): Akio Ebihara
Schools:
Gifu University
Yanagido, Gifu, Japan
Division:iGEMApplication Date:  2014-02-17
Section:Undergraduate
Region:AsiaAcceptance Date: 2014-04-23 12:49:42
Description:

Team Status
Welcome to iGEM 2014 ! Your iGEM 2014 team application was accepted by iGEM Headquarters on 2014-04-23 12:49:42 and your team registration fee has been received.
DNA Kit of Parts shipping information has been entered, thanks. View shipping information
Resource description has been submitted, thanks. View resource description
Your registration fee has been received. Thank you. More...

TrackMore...
Assigned Track: New Application

Title and AbstractMore...Download a .pdf version
Circular mRNA - the world's longest protein
Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive　one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming E.coli, we get world’s longest proteins.

Team Roster
Instructors
aebihara2014Akio Ebihara
isatoshiSatoshi IWAMOTO
h1884Hitoshi IWAHASHI
Student Members
ybannoYusuke Banno
nomukenNomura Kenta
momochiMomoko YAMADA
kozakaiKozakai Tomoya
MoriAkihiro Moriyama
Yuki1201Yuki Kamijo
SachiASachi Asano
takemaHasegawa Takema
fukufukuWataru Fukuda
LagomutryOuchi Ryo
uyuuEndo Yuki

This range of part numbers has been assigned to your team for use during the summer: BBa_K1332000 to BBa_K1332999