Difference between revisions of "Team:Waterloo"

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Engineered PAM Flexibility

Swappable sgRNA Targets

Antiviral Protection for Plants

CRISPR-Cas9 is an exciting tool for synthetic biologists because it can target and edit genomes with unprecedented specificity. Our team is attempting to re-engineer CRISPR to make it more flexible and easier to use.

We’re making it easy to test different sgRNA designs: restriction sites added to the sgRNA backbone allow 20 nucleotide target sequences to be swapped without excessive cloning.

Additionally, we’re applying recent research on viable mutations within Cas9’s PAM-interacting domain to design (d)Cas9 variants that bind to novel PAM sites, moving towards the goal of a suite of variants that can bind any desired sequence. We believe our re-engineered CRISPR-Cas9 will give biologists increased ability to optimize targeting in many applications.

The application we chose to explore is a proof-of-concept antiviral system defending the model plant Arabidopsis thaliana against Cauliflower Mosaic Virus, which would benefit from testing a large number of possible sgRNAs in the viral genome.

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+    <section>
+        <div class="small-cover" id="desc"><p>Description</p></div>
+        <div class="small-cover" id="medal"><p>Medal Requirements</p></div>
+        <div class="small-cover" id="team"><p>Team</p></div>
+        <div class="small-cover" id="att"><p>Attributions</p></div>
+        <div class="small-cover" id="collab"><p>Collaborations</p></div>
+    </section>
      <div class="prevHighlightBox">