Difference between revisions of "Team:Tokyo Tech/Experiment/RNA thermometer assay"
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<h3 id="Protocol" class="sub5">5.2. Assay Protocol</h3> | <h3 id="Protocol" class="sub5">5.2. Assay Protocol</h3> | ||
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| − | 1. | + | 1. Prepare 2 over night cultures for each sample in 3 mL LB medium containing chloramphenicol (25 microg / mL) at 37 ºC for 12 h.<br> |
2. コピペ。<br> | 2. コピペ。<br> | ||
3. コピペ。<br> | 3. コピペ。<br> | ||
Revision as of 01:07, 14 September 2015
RNA thermometer assay
We have characterized previous parts.
contents
1. Introduction
2. Summary of the Experiment
3. Results
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1. Protocol1
5.2.2. Protocol2
6. Reference
1. Introduction
ここにコピペ。
2. Summary of the Experiment
ここにコピペ。
3. Results
ここにコピペ。
4. Discussion
ここにコピペ。
5. Materials and Methods
5.1. Construction
-Strain
All the samples were DH5alpha strain.
-Plasmids
Device 1: J23101 + I13504(pSB1C3)
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Fig.3-7-4-1. |
5.2. Assay Protocol
1. Prepare 2 over night cultures for each sample in 3 mL LB medium containing chloramphenicol (25 microg / mL) at 37 ºC for 12 h.
2. コピペ。
3. コピペ。
4. コピペ。
5. コピペ。
6. コピペ。
7. コピペ。
8. コピペ。
9. コピペ。
6.. Reference
ここにコピペ。