Difference between revisions of "Team:Bielefeld-CeBiTec/Results/HeavyMetals"

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</br>
 
</br>
 
After finding out that the tested concentrations had no negative effect on <i> E. colis</i> growth we started <i> in vivo </i> characterization</br>
 
After finding out that the tested concentrations had no negative effect on <i> E. colis</i> growth we started <i> in vivo </i> characterization</br>
−
<h1 align="center"><i>in vivo</i></h1>
+
 
−
<h2>Arsenic</h2>
+
 
 +
<h1>Arsenic</h1>
 +
<h2><i>in vivo</i></h2>
 
<figure style="width: 600px">
 
<figure style="width: 600px">
 
<a href="https://static.igem.org/mediawiki/2015/1/1c/Bielefeld-CeBiTec_Arsenic_invivo_large.png" data-lightbox="heavymetals" data-title="Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations <i>in vivo</i>. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/2/2e/Bielefeld-CeBiTec_Arsenic_invivo_small.png" alt="Adjusting the detection limit"></a>
 
<a href="https://static.igem.org/mediawiki/2015/1/1c/Bielefeld-CeBiTec_Arsenic_invivo_large.png" data-lightbox="heavymetals" data-title="Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations <i>in vivo</i>. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/2/2e/Bielefeld-CeBiTec_Arsenic_invivo_small.png" alt="Adjusting the detection limit"></a>
 
<figcaption>Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations in vivo. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations in vivo. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
−
<h2>Chromium</h2>
+
 
 +
<h2><i>in vitro</i></h2>
 +
 
 +
<h1>Chromium</h1>
 +
 
 +
<h2><i>in vivo</i></h2>
 +
 
 
<figure style="width: 600px">
 
<figure style="width: 600px">
 
<a href="https://static.igem.org/mediawiki/2015/8/82/Bielefeld-CeBiTec_Biolector_chromium.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/8/82/Bielefeld-CeBiTec_Biolector_chromium.jpg" alt="Adjusting the detection limit"></a>
 
<a href="https://static.igem.org/mediawiki/2015/8/82/Bielefeld-CeBiTec_Biolector_chromium.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/8/82/Bielefeld-CeBiTec_Biolector_chromium.jpg" alt="Adjusting the detection limit"></a>
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<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
−
<h2>Copper</h2>
+
 
 +
<h2><i>in vitro</i></h2>
 +
 
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/9/99/Bielefeld-CeBiTec_Influence_of_chromium_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/9/99/Bielefeld-CeBiTec_Influence_of_chromium_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="http://https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_induction_chromium_in_chrB-cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_induction_chromium_in_chrB-cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
−
<h2>Lead</h2>
 
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="http://https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_Biolector_lead.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_Biolector_lead.jpg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_correction_induction_chromium_in_chrB-cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_correction_induction_chromium_in_chrB-cell-extract.jpeg" alt="Adjusting the detection limit"></a>
−
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
+
−
</figure>
+
−
<figure style="width: 600px">
+
−
<a href="https://static.igem.org/mediawiki/2015/a/aa/Bielefeld-CeBiTec_Biolector_lead_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/a/aa/Bielefeld-CeBiTec_Biolector_lead_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
+
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
−
 
−
<h2>Mercury</h2>
 
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/5/5b/Bielefeld-CeBiTec_mercury_flourescence-in_vivio.png" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/5/5b/Bielefeld-CeBiTec_mercury_flourescence-in_vivio.png" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/1/1d/Bielefeld-CeBiTec_induction_chromium_in_chrB_optimized_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/1/1d/Bielefeld-CeBiTec_induction_chromium_in_chrB_optimized_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/5/52/Bielefeld-CeBiTec_mercury_fluorescence_in_vivo_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/5/52/Bielefeld-CeBiTec_mercury_fluorescence_in_vivo_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/f/fe/Bielefeld-CeBiTec_Corr-induction-Cr-in-ChrBopt-CE.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/f/fe/Bielefeld-CeBiTec_Corr-induction-Cr-in-ChrBopt-CE.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
  
−
<h2>Nickel</h2>
+
<h1>Copper</h1>
−
<figure style="width: 600px">
+
 
−
<a href="https://static.igem.org/mediawiki/2015/c/c9/Bielefeld-CeBiTec_Biolector_nickel.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/c9/Bielefeld-CeBiTec_Biolector_nickel.jpg" alt="Adjusting the detection limit"></a>
+
<h2><i>in vivo</i></h2>
 +
 
 +
<figure style="width: 600px">
 +
<a href="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="http://https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
−
<h1 align="center"><i>in vitro</i></h1>
+
<h2><i>in vitro</i></h2>
−
<h2>Arsenic</h2>
+
−
<h2>Chromium</h2>
+
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/9/99/Bielefeld-CeBiTec_Influence_of_chromium_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/9/99/Bielefeld-CeBiTec_Influence_of_chromium_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
 +
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_induction_chromium_in_chrB-cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/d/d0/Bielefeld-CeBiTec_induction_chromium_in_chrB-cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_correction_induction_chromium_in_chrB-cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/1/1e/Bielefeld-CeBiTec_correction_induction_chromium_in_chrB-cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/1/1d/Bielefeld-CeBiTec_induction_chromium_in_chrB_optimized_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/1/1d/Bielefeld-CeBiTec_induction_chromium_in_chrB_optimized_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/f/fe/Bielefeld-CeBiTec_Corr-induction-Cr-in-ChrBopt-CE.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/f/fe/Bielefeld-CeBiTec_Corr-induction-Cr-in-ChrBopt-CE.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
−
 
−
<h2>Copper</h2>
 
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/3/37/Bielefeld-CeBiTec_Influence_of_copper_on_the_cell_extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
  
−
<figure style="width: 600px">
+
<h1>Lead</h1>  
−
<a href="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/cf/Bielefeld-CeBiTec_Abnahme_unter_Kupfer.jpeg" alt="Adjusting the detection limit"></a>
+
−
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
+
−
</figure>
+
  
 +
<h2><i>in vivo</i></h2>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/45/Bielefeld-CeBiTec_induction_copper_in_CueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="http://https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_Biolector_lead.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/d/d5/Bielefeld-CeBiTec_Biolector_lead.jpg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4c/Bielefeld-CeBiTec_correction_induction_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/a/aa/Bielefeld-CeBiTec_Biolector_lead_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/a/aa/Bielefeld-CeBiTec_Biolector_lead_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
  
−
<figure style="width: 600px">
+
<h1>Mercury</h1>
−
<a href="http://https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
−
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
+
−
</figure>
+
−
 
+
  
 +
<h2><i>in vivo</i></h2>
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/ce/Bielefeld-CeBiTec_induction_T7-copAP_copper_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/5/5b/Bielefeld-CeBiTec_mercury_flourescence-in_vivio.png" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/5/5b/Bielefeld-CeBiTec_mercury_flourescence-in_vivio.png" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
−
 
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
−
<a href="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/0/01/Bielefeld-CeBiTec_correction_induction_T7-copAP_in_cueR_cell-extract.jpeg" alt="Adjusting the detection limit"></a>
+
<a href="https://static.igem.org/mediawiki/2015/5/52/Bielefeld-CeBiTec_mercury_fluorescence_in_vivo_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/5/52/Bielefeld-CeBiTec_mercury_fluorescence_in_vivo_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
</figure>
 
</figure>
  
−
 
+
<h2><i>in vitro</i></h2>
−
<h2>Mercury</h2>
+
  
 
<figure style="width: 600px">
 
<figure style="width: 600px">
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<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
 
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<h1>Nickel</h1>
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<h2><i>in vivo</i></h2>
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<figure style="width: 600px">
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<a href="https://static.igem.org/mediawiki/2015/c/c9/Bielefeld-CeBiTec_Biolector_nickel.jpg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/c/c9/Bielefeld-CeBiTec_Biolector_nickel.jpg" alt="Adjusting the detection limit"></a>
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<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
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</figure>
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<figure style="width: 600px">
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<a href="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="TEXT Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/41/Bielefeld-CeBiTec_Biolector_nickel_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a>
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<figcaption>TEXT. Error bars represent the standard deviation of three biological replicates.</figcaption>
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Revision as of 03:58, 14 September 2015

iGEM Bielefeld 2015


Heavy Metals

Zusammenfassung in ganz wenigen Worten.


The different sensors we worked with were characterized in vivo as well as in vitro.

We tested the influence of each heavy metal on our sensors in vivo Therefore we used heavy metal concentrations based on heavy metal occurrences measured all over the world.
Adjusting the detection limit
Influence of heavy metals on the growth of E.coli KRX shown is the standard deviation of three biological replicates. For induction concentrations of 20 µg/L lead, 60 µg/L mercury, 60 µg/L chromium, 80 µg/L nickel, 40 mg/L copper which represent ten times of the WHO guideline were used.

After finding out that the tested concentrations had no negative effect on E. colis growth we started in vivo characterization

Arsenic

in vivo

Adjusting the detection limit
Time course of the induction of an arsenic biosensor with RFP for different arsenic concentrations in vivo. Error bars represent the standard deviation of three biological replicates.

in vitro

Chromium

in vivo

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

in vitro

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Copper

in vivo

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

in vitro

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Lead

in vivo

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Mercury

in vivo

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

in vitro

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

Nickel

in vivo

Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.
Adjusting the detection limit
TEXT. Error bars represent the standard deviation of three biological replicates.

And the date rape drugs?