Difference between revisions of "Team:Bielefeld-CeBiTec/Results/HeavyMetals"
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Our sensor for copper detection consists of CueR a MerR like activator and the copper specific promoter CopAP. The promoter is regulated by the CueR, which binds Cu-ions. We also used a sfGFP behind the promoter for detection trough a fluorescence signal. </br></br> | Our sensor for copper detection consists of CueR a MerR like activator and the copper specific promoter CopAP. The promoter is regulated by the CueR, which binds Cu-ions. We also used a sfGFP behind the promoter for detection trough a fluorescence signal. </br></br> | ||
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
| − | <a href="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" data-lightbox="heavymetals" data-title=" | + | <a href="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" data-lightbox="heavymetals" data-title="Time course of the induction of a chromium biosensor with sfGFP for different chromium concentrations in vivo. The data are measured with BioLector and normalized on OD600. Error bars represent the standard deviation of two biological replicates."><img src="https://static.igem.org/mediawiki/2015/9/90/Bielefeld-CeBiTec_Biolector_copper.jpg" alt="Adjusting the detection limit"></a> |
| − | <figcaption> | + | <figcaption>Time course of the induction of a chromium biosensor with sfGFP for different chromium concentrations in vivo. The data are measured with BioLector and normalized on OD600. Error bars represent the standard deviation of two biological replicates.</figcaption> |
</figure></br></br> | </figure></br></br> | ||
| − | <i>In vivo</i> we could show that the adding different concentrations of | + | <i>In vivo</i> we could show that the adding different concentrations of copper has different effects on the transcription of sfGFP. </br></br> |
<figure style="width: 600px"> | <figure style="width: 600px"> | ||
| − | <a href="http://https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title=" | + | <a href="http://https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" data-lightbox="heavymetals" data-title="Fluorescence levels at three different stages of cultivation. Shown are levels after 60 minutes, 150 minutes and 650 minutes. Error bars represent the standard deviation of three biological replicates."><img src="https://static.igem.org/mediawiki/2015/4/4e/Bielefeld-CeBiTec_Biolector_copper_Balkendiagramm.jpeg" alt="Adjusting the detection limit"></a> |
| − | <figcaption> | + | <figcaption>Fluorescence levels at three different stages of cultivation. Shown are levels after 60 minutes, 150 minutes and 650 minutes. .</figcaption> |
</figure> | </figure> | ||
Revision as of 04:38, 14 September 2015
Heavy Metals
Zusammenfassung in ganz wenigen Worten.
Arsenic
in vivo
in vitro
Chromium
in vivo
Our sensor for chromium detection consists of ChrB the repressor and the chromate specific promoter ChrP. The promoter is regulated by the ChrB, which binds Cr-ions. Behind the promoter is a sfGFP for detection of a fluorescence signal. In vivo we could show that the addition of different concentrations of chromium have different effects to transcription of sfGFP.
in vitro
Copper
in vivo
Our sensor for copper detection consists of CueR a MerR like activator and the copper specific promoter CopAP. The promoter is regulated by the CueR, which binds Cu-ions. We also used a sfGFP behind the promoter for detection trough a fluorescence signal.
in vitro
Lead
in vivo
Mercury
in vivo
in vitro
Nickel
in vivo
