Difference between revisions of "Team:UFSCar-Brasil/part3.html"
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<h3 class="ui header" id="overview">Curve fitting</h3> | <h3 class="ui header" id="overview">Curve fitting</h3> | ||
<p> Based on laboratory experiments was possible to plotting fluorescence standard curve as a function of GFP concentration (Green Fluorescent Protein). This adjustment was essential to facilitated the process of obtaining the GFP molecules number just using the fluorescence values obtained in lab. </p> | <p> Based on laboratory experiments was possible to plotting fluorescence standard curve as a function of GFP concentration (Green Fluorescent Protein). This adjustment was essential to facilitated the process of obtaining the GFP molecules number just using the fluorescence values obtained in lab. </p> | ||
| − | <p>The curve that better fitted the fluorescence standard curve points was the straight: | + | <p>The curve that better fitted the fluorescence standard curve points was the straight: |
| − | + | (1) $$ F_{luo}=a[GFP]+b $$ | |
<p> Where the parameters a and b are constants, [GFP] is GFP concentrations given by nM and F_luo is fluorescence in u.a. </p> | <p> Where the parameters a and b are constants, [GFP] is GFP concentrations given by nM and F_luo is fluorescence in u.a. </p> | ||
Revision as of 15:17, 14 September 2015
Part III
Frase de impacto
Curve fitting
Based on laboratory experiments was possible to plotting fluorescence standard curve as a function of GFP concentration (Green Fluorescent Protein). This adjustment was essential to facilitated the process of obtaining the GFP molecules number just using the fluorescence values obtained in lab.
The curve that better fitted the fluorescence standard curve points was the straight: (1) $$ F_{luo}=a[GFP]+b $$
Where the parameters a and b are constants, [GFP] is GFP concentrations given by nM and F_luo is fluorescence in u.a.
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