We constructed biosensors for date rape drugs, copper and mercury that work in an lyophilized in vitro transcription/translation system applied to paper. We decided to work with two different cell-free systems, both were functional. The fluorescence output could be analyzed by our newly developed filter system with a simple smartphone app. Since working with date rape drugs is an ... issue, we decided to put a great effort into the human practices aspect of our project by creating a report on the topic of "dual use", in order to prevent spreading of misusable information and fathom the limits of open source ....

We were able to establish a new assay called the plasmid repressor interaction assay (PRIA) for the model system LacI-lacO. For the implementation of the assay on paper we immobilized amino- and Cy3-labeled DNA containing the operator site successfully on filter paper. We were able to prove with an electrophoretic mobility shift assay that the repressor proteins (ArsR, LacI and BlcR) fused with sfGFP we used can bind to oligonucleotides with the corresponding operator site (arsO, lacO, P_blc). For the detection of the fusion protein and Cy3-labeled DNA we were able to exhibit fluorescence of them both with the Ettan DIGE scanner.

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