Difference between revisions of "Team:Bielefeld-CeBiTec/Design"
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| − | <p> Therefore we bought a lee color filter catalog and began to test different filter combinations. But before testing we decided to messure the extinction and emission spektra from sfGFP as a basis to choose the most promising filter | + | <p> Therefore we bought a lee color filter catalog and began to test different filter combinations. But before testing we decided to messure the extinction and emission spektra from sfGFP as a basis to choose the most promising filter combination and to have a look if the smartphone flash really extincts sfGFP.The preselection was possible, because every Filter had a light transmitting spektra. </p> |
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| + | <a href="https://static.igem.org/mediawiki/2015/3/36/Bielefeld-CeBiTec_sfGFP_Spektra.png" data-lightbox="detection" data-title=" Emission and extinction spektra of sfGFP. The characteristic curve from the samsung galaxy S5 mini flash is also shown. The overlap from the different spektra show, it's possible to excite sfGFP with a smartphone flash. "><img src="https://static.igem.org/mediawiki/2015/3/36/Bielefeld-CeBiTec_sfGFP_Spektra.png" ></a> | ||
| + | <figcaption>Emission and extinction spektra of sfGFP. The characteristic curve from the samsung galaxy S5 mini flash is also shown. The overlap from the different spektra show, it's possible to excite sfGFP with a smartphone flash. | ||
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<p> The next step was to create an dark environment. Therefore we did handicrafts to design a "black box". Now it was possible to take photos under same conditions and same background. </p> | <p> The next step was to create an dark environment. Therefore we did handicrafts to design a "black box". Now it was possible to take photos under same conditions and same background. </p> | ||
Revision as of 16:45, 14 September 2015
Prototype Design
The first steps
In the beginning we wanted to test, if it could really work to photograph fluorescence with an smartphone. Therefore we got an light engineering Filter from the "Frauenkulturzentrum Bielefeld e.V." and put it in front of the camera and the flash. You can see different colors in the picture. So in our first impression we thought it's worth to try some other Filters.
Therefore we bought a lee color filter catalog and began to test different filter combinations. But before testing we decided to messure the extinction and emission spektra from sfGFP as a basis to choose the most promising filter combination and to have a look if the smartphone flash really extincts sfGFP.The preselection was possible, because every Filter had a light transmitting spektra.
The next step was to create an dark environment. Therefore we did handicrafts to design a "black box". Now it was possible to take photos under same conditions and same background.
The filter combinitions
The groundwork is done. Now we had to find the ideal Filterkombination. So we made a lot of photos with the preselection in every combinition. After analyzing the photos with Fiji. The optimal filter combination was tokyo blue in front of the flash and twickenham green in front of the camera.
As you can see in the picture above it's really important to take the right filters. But is it only possible to photograph sfGFP and GFP? To find out, we tried to photograph monomeric red fluorescent protein (mRFP) Lysat.
