Difference between revisions of "Team:TU Delft/Hardware"
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<p class="lead">We built a functional prototype of a 3D printer, our Biolinker, from cheap DIY open source parts, which is capable of bioprinting bacteria in shapes based on lines and circles. The bioink is made out of our engineered bacterial cells, which we successfully immobilized into a gel. With this bioink we can print lines with a resolution (thickness) of down to 1 mm. We were able to successfully print and image of up to 4 layers of bioink with only little mixing between the layers. Although redissolving jellified alginate becomes more challenging with size, our bacterial cells were able to survive under alginate dissolving conditions for 24 hours.</p> | <p class="lead">We built a functional prototype of a 3D printer, our Biolinker, from cheap DIY open source parts, which is capable of bioprinting bacteria in shapes based on lines and circles. The bioink is made out of our engineered bacterial cells, which we successfully immobilized into a gel. With this bioink we can print lines with a resolution (thickness) of down to 1 mm. We were able to successfully print and image of up to 4 layers of bioink with only little mixing between the layers. Although redissolving jellified alginate becomes more challenging with size, our bacterial cells were able to survive under alginate dissolving conditions for 24 hours.</p> | ||
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| − | <img src="https://static.igem.org/mediawiki/2015/0/07/TU_Delft_Print04.jpeg"> | + | <img class="featurette-image img-responsive center-block" src="https://static.igem.org/mediawiki/2015/0/07/TU_Delft_Print04.jpeg" style="height:200px; padding-top:15px; margin-top:20px; background-size: cover;" alt="Generic placeholder image"> |
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| + | <p class="lead">Since the 1980s numerous applications and materials have been developed for 3D printing. Nowadays, starting from a computer generated three dimensional image file (stereolithography .STL) metals, plastics or even food can be printed in any shape imaginable. Recently, significant progress has been made in the three dimensional printing of mammalian stem cells towards creating artificial organs with many renowned research groups all around the world working on this. Since 3D bioprinting has high potential and is still largely unexplored, we set out to find a novel application. Inspired by machine-specific properties: precision and reproducibility, we came up with the idea to print cells in a structurally defined pattern. In this way we intend to meet the increasing demand of reliable product testing in medicine and health applications.</p> | ||
| + | <p class="lead">In the following pages, we describe the development and the printing conditions of our prototype.</p> | ||
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Revision as of 17:16, 14 September 2015
Hardware Title
Subtitle
Overview
The Biolinker - a DIY biofilm printer
We built a functional prototype of a 3D printer, our Biolinker, from cheap DIY open source parts, which is capable of bioprinting bacteria in shapes based on lines and circles. The bioink is made out of our engineered bacterial cells, which we successfully immobilized into a gel. With this bioink we can print lines with a resolution (thickness) of down to 1 mm. We were able to successfully print and image of up to 4 layers of bioink with only little mixing between the layers. Although redissolving jellified alginate becomes more challenging with size, our bacterial cells were able to survive under alginate dissolving conditions for 24 hours.
Since the 1980s numerous applications and materials have been developed for 3D printing. Nowadays, starting from a computer generated three dimensional image file (stereolithography .STL) metals, plastics or even food can be printed in any shape imaginable. Recently, significant progress has been made in the three dimensional printing of mammalian stem cells towards creating artificial organs with many renowned research groups all around the world working on this. Since 3D bioprinting has high potential and is still largely unexplored, we set out to find a novel application. Inspired by machine-specific properties: precision and reproducibility, we came up with the idea to print cells in a structurally defined pattern. In this way we intend to meet the increasing demand of reliable product testing in medicine and health applications.
In the following pages, we describe the development and the printing conditions of our prototype.
Making 3D Biofilms
Subtitle or summary goes here. Should be short - two or three sentences.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent ultrices tincidunt ipsum, vitae tempor nibh porta ac. Fusce consectetur neque et dolor vestibulum iaculis. Nunc pretium turpis at arcu tempus vehicula. Nam nec accumsan metus, ac tempus tortor. Aenean euismod elit vitae ex ultrices pulvinar. Etiam rhoncus non urna vel volutpat. Donec ut erat ornare, faucibus quam a, posuere urna. Phasellus at nisl sed erat ultricies commodo vel ut mauris. Morbi ac mauris dui. Cras sit amet ornare nisl. Suspendisse lectus mi, ullamcorper et dolor a, vulputate condimentum velit. Morbi dolor eros, cursus euismod magna sit amet, tempus volutpat quam. Morbi at est sed erat efficitur lobortis nec non elit. Integer urna nisi, dapibus nec magna non, pharetra sodales felis. Fusce dignissim elit sit amet purus aliquet, quis luctus tortor commodo. Donec viverra enim vel ultrices iaculis.
The Biolinker
Subtitle or summary goes here. Should be short - two or three sentences.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent ultrices tincidunt ipsum, vitae tempor nibh porta ac. Fusce consectetur neque et dolor vestibulum iaculis. Nunc pretium turpis at arcu tempus vehicula. Nam nec accumsan metus, ac tempus tortor. Aenean euismod elit vitae ex ultrices pulvinar. Etiam rhoncus non urna vel volutpat. Donec ut erat ornare, faucibus quam a, posuere urna. Phasellus at nisl sed erat ultricies commodo vel ut mauris. Morbi ac mauris dui. Cras sit amet ornare nisl. Suspendisse lectus mi, ullamcorper et dolor a, vulputate condimentum velit. Morbi dolor eros, cursus euismod magna sit amet, tempus volutpat quam. Morbi at est sed erat efficitur lobortis nec non elit. Integer urna nisi, dapibus nec magna non, pharetra sodales felis. Fusce dignissim elit sit amet purus aliquet, quis luctus tortor commodo. Donec viverra enim vel ultrices iaculis.
Design and Integration
Subtitle or summary goes here. Should be short - two or three sentences.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent ultrices tincidunt ipsum, vitae tempor nibh porta ac. Fusce consectetur neque et dolor vestibulum iaculis. Nunc pretium turpis at arcu tempus vehicula. Nam nec accumsan metus, ac tempus tortor. Aenean euismod elit vitae ex ultrices pulvinar. Etiam rhoncus non urna vel volutpat. Donec ut erat ornare, faucibus quam a, posuere urna. Phasellus at nisl sed erat ultricies commodo vel ut mauris. Morbi ac mauris dui. Cras sit amet ornare nisl. Suspendisse lectus mi, ullamcorper et dolor a, vulputate condimentum velit. Morbi dolor eros, cursus euismod magna sit amet, tempus volutpat quam. Morbi at est sed erat efficitur lobortis nec non elit. Integer urna nisi, dapibus nec magna non, pharetra sodales felis. Fusce dignissim elit sit amet purus aliquet, quis luctus tortor commodo. Donec viverra enim vel ultrices iaculis.
DIY Manuals
Subtitle or summary goes here. Should be short - two or three sentences.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent ultrices tincidunt ipsum, vitae tempor nibh porta ac. Fusce consectetur neque et dolor vestibulum iaculis. Nunc pretium turpis at arcu tempus vehicula. Nam nec accumsan metus, ac tempus tortor. Aenean euismod elit vitae ex ultrices pulvinar. Etiam rhoncus non urna vel volutpat. Donec ut erat ornare, faucibus quam a, posuere urna. Phasellus at nisl sed erat ultricies commodo vel ut mauris. Morbi ac mauris dui. Cras sit amet ornare nisl. Suspendisse lectus mi, ullamcorper et dolor a, vulputate condimentum velit. Morbi dolor eros, cursus euismod magna sit amet, tempus volutpat quam. Morbi at est sed erat efficitur lobortis nec non elit. Integer urna nisi, dapibus nec magna non, pharetra sodales felis. Fusce dignissim elit sit amet purus aliquet, quis luctus tortor commodo. Donec viverra enim vel ultrices iaculis.
Protocols
Subtitle or summary goes here. Should be short - two or three sentences.
Lorem ipsum dolor sit amet, consectetur adipiscing elit. Praesent ultrices tincidunt ipsum, vitae tempor nibh porta ac. Fusce consectetur neque et dolor vestibulum iaculis. Nunc pretium turpis at arcu tempus vehicula. Nam nec accumsan metus, ac tempus tortor. Aenean euismod elit vitae ex ultrices pulvinar. Etiam rhoncus non urna vel volutpat. Donec ut erat ornare, faucibus quam a, posuere urna. Phasellus at nisl sed erat ultricies commodo vel ut mauris. Morbi ac mauris dui. Cras sit amet ornare nisl. Suspendisse lectus mi, ullamcorper et dolor a, vulputate condimentum velit. Morbi dolor eros, cursus euismod magna sit amet, tempus volutpat quam. Morbi at est sed erat efficitur lobortis nec non elit. Integer urna nisi, dapibus nec magna non, pharetra sodales felis. Fusce dignissim elit sit amet purus aliquet, quis luctus tortor commodo. Donec viverra enim vel ultrices iaculis.