contents

1. Introduction?

2．The original Prisoner’s Dilemma scenario in game theory

3. Replication of the payoff matrix using E. coli

3.1 Prisoner coli’s dilemma payoff matrix

3.1.1 Both cooperation leads to low damage

3.1.2 Both defection leads to middle damage

3.1.3 When one is fooled, two types of damage

3.2 Modeling selected a solution for leaky expression problem to satisfy the requirement of the payoff matrix

3.3 Improvementof CmR protein property by addition of ssrA degradation tag

3.4 Succeeded to replicate the payoff matrix through wet lab

4. Prisoner coli decides its option : Cooperate or Defect

4.1 How to decide?

4.2 Decision making coli

5. Iterated game with several strategies

5.1 The four strategies

5.1.1 Random strategy

5.1.2 Always cooperate strategy

5.1.3 Always defect strategy

5.1.4 Tit-for-tat strategy

5.1.4.1 FimE assay: recombinase that inverts fim switch in only one way

6. Dilemma game in general public

7. Reference

1. Introduction

We decided to replay the Prisoner’s Dilemma game, in which one’s profit depend on options of cooperation and defection, by using E. coli. The Prisoner’s Dilemma, a well-known game analyzed in game theory, involves dilemma between cooperation and defection. To pursue his or her own profit, one will choose to cooperate or to defect in the game.
In our E. coli’s version of Prisoner’s Dilemma game involving two prisoner coli, the results of their options define the profit they obtained. Here, profit means the growth of E. coli. Like the prisoners in the game theory, we genetically engineered two E. coli to act as the prisoners, Prisoner A and Prisoner B. The prisoner colis are able to cooperate or to defect in our game. The combinations of their options (cooperation or defection) affect the profit they obtained which equals to their growth. To cooperate, they produce AHL while to defect, they do not produce AHL. Each prisoner coli is designed to produce different type of AHL (C4HSL or 3OC12HSL). The act of producing AHL imposes a metabolic burden on both prisoner coli.