Difference between revisions of "Team:Tokyo Tech/Experiment/Overview of fim inversion system"

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       <h3 class="link2"><a href="#Wild">2.1. Wild type <i>fim</i>&nbsp;switch</a></h3>
 
       <h3 class="link2"><a href="#Wild">2.1. Wild type <i>fim</i>&nbsp;switch</a></h3>
 
         <h3 class="link3"><a href="#tdesign">2.1.1. The design of wild type <i>fim</i>&nbsp;switch </a></h3>
 
         <h3 class="link3"><a href="#tdesign">2.1.1. The design of wild type <i>fim</i>&nbsp;switch </a></h3>
         <h3 class="link3"><a href="#FimB/FimE">2.1.2. Result of FimB/FimE Assay to fimS(TT)</a></h3>
+
         <h3 class="link3"><a href="#wFimB/FimE">2.1.2. Result of FimB/FimE Assay to fimS(TT)</a></h3>
 
         <h3 class="link3"><a href="#AHL">2.1.3. AHL dependent FimE Assay to fimS(TT)</a></h3>
 
         <h3 class="link3"><a href="#AHL">2.1.3. AHL dependent FimE Assay to fimS(TT)</a></h3>
 
       <h3 class="link2"><a href="#Tokyofim">2.2. Tokyo_Tech <i>fim</i>&nbsp;switch</a></h3>
 
       <h3 class="link2"><a href="#Tokyofim">2.2. Tokyo_Tech <i>fim</i>&nbsp;switch</a></h3>
 
         <h3 class="link3"><a href="#wdesign">2.2.1. The design of Tokyo_Tech <i>fim</i>&nbsp;switch </a></h3>
 
         <h3 class="link3"><a href="#wdesign">2.2.1. The design of Tokyo_Tech <i>fim</i>&nbsp;switch </a></h3>
         <h3 class="link3"><a href="#FimB">2.2.2. Result of FimB/FimE Assay to fimS(WT)</a></h3>
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         <h3 class="link3"><a href="#tFimB/FimE">2.2.2. Result of FimB/FimE Assay to fimS(WT)</a></h3>
  
  
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               <h3 id="AHL" class="sub6">2.1.3. AHL dependent FimE Assay to fimS(TT)</h3>
 
               <h3 id="AHL" class="sub6">2.1.3. AHL dependent FimE Assay to fimS(TT)</h3>
 
               <h3 id="Tokyofim" class="sub5">2.2. Tokyo_Tech <i>fim</i>&nbsp;switch</h3>
 
               <h3 id="Tokyofim" class="sub5">2.2. Tokyo_Tech <i>fim</i>&nbsp;switch</h3>
               <h3 id="tdesign" class="sub6">2.2.1. The design of wild type <i>fim</i>&nbsp;switch</h3>
+
               <h3 id="wdesign" class="sub6">2.2.1. The design of wild type <i>fim</i>&nbsp;switch</h3>
               <h3 id="FimB/FimE" class="sub6">2.2.2. Result of FimB/FimE Assay to fimS(TT)</h3>
+
               <h3 id="tFimB/FimE" class="sub6">2.2.2. Result of FimB/FimE Assay to fimS(TT)</h3>
  
  

Revision as of 03:25, 15 September 2015

Overview of fim inversion system

We introduce fim system

  
  

1. Introduction

      

ここにコピペ。

2. How to fim switch works

2.1. Wild type fim switch

2.1.1. The design of wild type fim switch

2.1.2. Result of FimB/FimE Assay to fimS(TT)

2.1.3. AHL dependent FimE Assay to fimS(TT)

2.2. Tokyo_Tech fim switch

2.2.1. The design of wild type fim switch

2.2.2. Result of FimB/FimE Assay to fimS(TT)

2.2. Wild type fim switch

3. Results

      

ここにコピペ。

4. Discussion

      

ここにコピペ。

5. Materials and Methods

5.1. Construction

-Strain

      

All the samples were DH5alpha strain.

-Plasmids

      

Device 1: J23101 + I13504(pSB1C3)

Fig.3-7-4-1.


5.2. Assay Protocol

5.2.1. Protocol1

1. コピペ。
2. コピペ。
3. コピペ。
4. コピペ。
5. コピペ。
6. コピペ。
7. コピペ。
8. コピペ。
9. コピペ。

6.. Reference

      

ここにコピペ。