Difference between revisions of "Team:Tokyo Tech/Experiment/Overview of fim inversion system"

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           <h2 id="Introduction" class="smalltitle">1. Introduction</h2>
 
           <h2 id="Introduction" class="smalltitle">1. Introduction</h2>
 
　　　　　　<p class="text">ここにコピペ。</p>
 
　　　　　　<p class="text">ここにコピペ。</p>
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      <td width="940px"><div align="center"><img src="https://2015.igem.org/File:Tokyo_Tech_fim_over_view1.png" />
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      </td>
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      </tr>
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      <tr>
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      <td width="940px">
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      <h4 align="center" class="fig"><strong>Fig.3-3-3-1.</strong>&nbsp;ここに図の名前を打て</h4>
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      <td>
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      </tr>
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      </table>
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　　　　　　<p class="text">In order to make the E. coli decide for themselves, we needed a part that can switch back and forth at random. We decided that the fim switch can do this job.cccffcffcfffffff7
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Our big achievement have created a tripartite relationship, fim switch-FimB/FimE.
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</p>
  
 
           <h2 id="How" class="smalltitle">2. How to <i>fim</i> switch works</h2>
 
           <h2 id="How" class="smalltitle">2. How to <i>fim</i> switch works</h2>

Revision as of 04:11, 15 September 2015

Overview of fim inversion system

We introduce fim system

　　

contents

1. Introduction

2. How to fim switch works

2.1. Wild type fim switch

2.1.1. The design of wild type fim switch

2.1.2. Result of FimB/FimE Assay to fimS(TT)

2.1.3. AHL dependent FimE Assay to fimS(TT)

2.2. Tokyo_Tech fim switch

2.2.1. The design of Tokyo_Tech fim switch

2.2.2. Result of FimB/FimE Assay to fimS(WT)

3. Comparison other team’s fim switch

3.1. FimB(2013_Toronto)

3.2. Fim switch(2006_Caltech)-FimE(2006_Caltech)

3.3. Fim switch(2013_Michigan)-FimE(2006_Caltech)-HbiF(2012_Michigan)

4. Reference


　　

1. Introduction

　　　　　　

ここにコピペ。

Fig.3-3-3-1. ここに図の名前を打て
　　　　　　

In order to make the E. coli decide for themselves, we needed a part that can switch back and forth at random. We decided that the fim switch can do this job.cccffcffcfffffff7 Our big achievement have created a tripartite relationship, fim switch-FimB/FimE.

2. How to fim switch works

2.1. Wild type fim switch

2.1.1. The design of wild type fim switch

2.1.2. Result of FimB/FimE Assay to fimS(TT)

2.1.3. AHL dependent FimE Assay to fimS(TT)

2.2. Tokyo_Tech fim switch

2.2.1. The design of wild type fim switch

2.2.2. Result of FimB/FimE Assay to fimS(TT)

3. Comparison other team’s fim switch

3.1. FimB(2013_Toronto)

　　　　　　

3.2. Fim switch(2006_Caltech)-FimE(2006_Caltech)

　　　　　　

3.3. Fim switch(2013_Michigan)-FimE(2006_Caltech)-HbiF(2012_Michigan)

　　　　　　

4. Reference