Difference between revisions of "Team:Bielefeld-CeBiTec/Organisms"
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| − | + | <h2><i>Escherichia coli</i></h2> | |
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| − | <p> We used following strains of <i>E. coli</i> during our project:</p> | + | |
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| + | <p> We used following strains of <i>E. coli</i> during our project:</p> | ||
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| + | <li> KRX </li> | ||
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<li> Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] </li> | <li> Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm] </li> | ||
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| + | <h2><i>Bacillus subtilis</i></h2> | ||
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| + | <p> In our project we used <i>B. subtilis</i> strain 168 with genotype <i>trpC2</i>. You can find further information about the origin of this strain in <a href="#Zeigler2008">Zeigler et al.</a></p> | ||
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| − | + | <h2><i>Agrobacterium tumefaciens</i></h2> | |
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| − | <p> We isolated some sequence from <i>A. tumefaciens</i> C58.1. For detailed information about this organism please be referred to <a href="#Wood2001">Wood et al. 2001</a>. </p> | + | |
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| + | <p> We isolated some sequence from <i>A. tumefaciens</i> C58.1. For detailed information about this organism please be referred to <a href="#Wood2001">Wood et al. 2001</a>. </p> | ||
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Revision as of 13:45, 15 September 2015
Organisms
...
Escherichia coli
We used following strains of E. coli during our project:
- KRX
- Genotype: [F´, traD36, ΔompP, proA+B+, lacIq, Δ(lacZ)M15] ΔompT, endA1, recA1, gyrA96 (Nalr), thi-1, hsdR17 (rk–, mk+), e14– (McrA–), relA1, supE44, Δ(lac-proAB), Δ(rhaBAD)::T7 RNA polymerase.
- ER2566 (T7 Express Competent E. coli)
- Genotype: F- λ- fhuA2 [lon] ompT lacZ::T7 gene 1 gal sulA11 Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10-TetS)2 R(zgb-210::Tn10)(TetS) endA1 [dcm]
Bacillus subtilis
In our project we used B. subtilis strain 168 with genotype trpC2. You can find further information about the origin of this strain in Zeigler et al.
Agrobacterium tumefaciens
We isolated some sequence from A. tumefaciens C58.1. For detailed information about this organism please be referred to Wood et al. 2001.
References
Wood, D. W.; Setubal, J. C.; Kaul, R.; Monks, D. E.; Kitajima, J. P.; Okura, V. K. et al. (2001): The genome of the natural genetic engineer Agrobacterium tumefaciens C58. In: Science (New York, N.Y.) 294 (5550), S. 2317–2323. DOI: 10.1126/science.1066804.
Zeigler, Daniel R.; Prágai, Zoltán; Rodriguez, Sabrina; Chevreux, Bastien; Muffler, Andrea; Albert, Thomas et al. (2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. In: Journal of bacteriology 190 (21), S. 6983–6995. DOI: 10.1128/JB.00722-08.
