Difference between revisions of "Team:Yale/notebook"

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     <div id="week1" data-reveal="" aria-labelledby="iGEM Modal" aria-hidden="true" role="dialog" class="reveal-modal grayModal">
 
     <div id="week1" data-reveal="" aria-labelledby="iGEM Modal" aria-hidden="true" role="dialog" class="reveal-modal grayModal">
 
       <h2 class="modal__title">Lessons from Week -1</h2>
 
       <h2 class="modal__title">Lessons from Week -1</h2>
      <p>Today, we're visiting a museum. Hello!</p>
 
      <p class="text-center"><img src="http://client.cameronyick.us/igem/assets/img/journal/pigeon.jpg"></p>
 
 
       <p>Dan began his research for the summer two weeks before the other researchers arrived. This was the first week any iGEM researcher was in the lab, so it was focused on preparing the lab and assessing the viability of two of our possible projects. The project which was started this week focused on porting Multiplex Automated Genome Engineering (MAGE) from E. Coli, in which the technique is proven to work, to Cyanobacteria and Sinorhizobium bacteria.</p>
 
       <p>Dan began his research for the summer two weeks before the other researchers arrived. This was the first week any iGEM researcher was in the lab, so it was focused on preparing the lab and assessing the viability of two of our possible projects. The project which was started this week focused on porting Multiplex Automated Genome Engineering (MAGE) from E. Coli, in which the technique is proven to work, to Cyanobacteria and Sinorhizobium bacteria.</p>
 
       <p>In order to port MAGE from E. Coli to other organisms, we had to first find inducible promoters that worked in Cyanobacteria and Sinorhizobia, and then beta-homolog proteins (reference to MAGE section of our wiki) for both organisms. At this point in the project, we began with finding cyanobacterial inducible promoters. We found promoters in Cyanobacteria which should, in theory, be inducible, and one constitutive promoter. The promoters were a nitrate-inducible promoter nirA, an iron-inducible promoter isiAB, a green-light-inducible promoter pCpG2, and a constitutive promoter, psaA. We designed and ordered primers to isolate all of these promoters from their respective backbones, whether they were Synechococcus sp. 7002 or Synechocystis sp. 6803. We also focused our efforts on growing up copies of a backbone plasmid, BBa_K125000, which was to contain our primers.</p>
 
       <p>In order to port MAGE from E. Coli to other organisms, we had to first find inducible promoters that worked in Cyanobacteria and Sinorhizobia, and then beta-homolog proteins (reference to MAGE section of our wiki) for both organisms. At this point in the project, we began with finding cyanobacterial inducible promoters. We found promoters in Cyanobacteria which should, in theory, be inducible, and one constitutive promoter. The promoters were a nitrate-inducible promoter nirA, an iron-inducible promoter isiAB, a green-light-inducible promoter pCpG2, and a constitutive promoter, psaA. We designed and ordered primers to isolate all of these promoters from their respective backbones, whether they were Synechococcus sp. 7002 or Synechocystis sp. 6803. We also focused our efforts on growing up copies of a backbone plasmid, BBa_K125000, which was to contain our primers.</p>

Revision as of 01:10, 16 September 2015


<!DOCTYPE html> Yale iGem 2015: Notebook

Lab Notebook