Difference between revisions of "Team:Yale/notebook"

Line 147: Line 147:
 
     <div id="week5" data-reveal="" aria-labelledby="iGEM Modal" aria-hidden="true" role="dialog" class="reveal-modal grayModal">
 
     <div id="week5" data-reveal="" aria-labelledby="iGEM Modal" aria-hidden="true" role="dialog" class="reveal-modal grayModal">
 
       <h2 class="modal__title">Lessons from Week 4</h2>
 
       <h2 class="modal__title">Lessons from Week 4</h2>
       <p>     This week, we made progress in amplifying cyanobacteria and rhizobium promoters. We performed multiple antibiotic resistance assays and transformation experiments in both types of bacteria as part of our attempt to create a framework for propagating and selecting nonmodel organisms. </p>
+
       <p>This week, we made progress in amplifying cyanobacteria and rhizobium promoters. We performed multiple antibiotic resistance assays and transformation experiments in both types of bacteria as part of our attempt to create a framework for propagating and selecting nonmodel organisms. </p>
 
       <p>All of the primers for our cyanobacteria promoters have arrived. Dan worked on amplifying them so that they can be assembled with citrine into one of the vectors we have and tested for strength. Unfortunately, due to a power outage that halted the PCR run, no amplifications were successful.</p>
 
       <p>All of the primers for our cyanobacteria promoters have arrived. Dan worked on amplifying them so that they can be assembled with citrine into one of the vectors we have and tested for strength. Unfortunately, due to a power outage that halted the PCR run, no amplifications were successful.</p>
 
       <p>One of the bigger issues we handled this week was the cyanobacteria growth media. We ordered freshwater BG-11 from Sigma-Aldrich, but we needed marine BG-11 to grow PCC 7002. We researched and found the reagents necessary to prepare MN marine medium from a seawater base in our lab.</p>
 
       <p>One of the bigger issues we handled this week was the cyanobacteria growth media. We ordered freshwater BG-11 from Sigma-Aldrich, but we needed marine BG-11 to grow PCC 7002. We researched and found the reagents necessary to prepare MN marine medium from a seawater base in our lab.</p>

Revision as of 12:38, 16 September 2015


<!DOCTYPE html> Yale iGem 2015: Notebook

Lab Notebook