Difference between revisions of "Team:EPF Lausanne/Judging"
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<td>Improve the function or characterization of a previously existing BioBrick Part or Device</td> | <td>Improve the function or characterization of a previously existing BioBrick Part or Device</td> | ||
| − | <td></td> | + | <td><a href="http://www.ncbi.nlm.nih.gov/pubmed/23360965" target="blank">Bikard et al.</a> used dCas9-\(\omega\) in order to regulate gene expression. Their dCas9 system works with tracRNAs, while our system works with simpler gRNAs. In order to use our dCas9-gRNA system we had to modify Bikard's promoter. In addition, to test new gRNA sequences we created a modified synthetic version of this promoter.</td> |
| − | <td></td> | + | <td><a href="http://parts.igem.org/Part:BBa_K1723000" target="blank">BBa_K1723000</a></br> |
| + | <a href="http://parts.igem.org/Part:BBa_K1723005" target="blank">BBa_K1723001</a></br> | ||
| + | <a href="http://parts.igem.org/Part:BBa_K1723005" target="blank">BBa_K1723005</a></td> | ||
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Revision as of 21:08, 16 September 2015
Take a look at our judging form for more details!
Medal criteria
Bronze medal
| Criterion | Description | Link |
|---|---|---|
| Register for iGEM, have a great summer, and attend the Giant Jamboree. | The team from EPFL (Ecole Polythencique Fédérale de Lausanne) is successful registered to iGEM, the premiere student competition in Synthetic Biology. | EPF_Lausanne |
| Complete judging form | The judging form helps iGEM judges to find important informations in order to equally evaluate each team. | Judging form |
| Create and share a Description of the team's project using the iGEM wiki. | All the work done by the EPFL iGEM team is documented in this website, called Wiki because powered by Mediawiki. Have a look! | Wiki |
| Present a poster and a talk at the iGEM Jamboree. | EPFL iGEM team will fly in Boston on Septenber 24 in order to present the project to other teams coming from all around the world and to discuss it with the judges. | |
| Create a page on your team wiki with clear attribution of each aspect of your project. | An iGEM team is often composed of several students with different backgrounds and they are helped by PhD students or professors. The work is attributed clearly to each person which contributed to the project. | Attributions |
| Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry | For S. cerevisiae we created new BioBricks. They consists on dCas9 fused with the VP64 RNA polymerase subunit and different synthetic gRNAs. | BBa_K1723021 BBa_K1723009 BBa_K1723010 BBa_K1723011 BBa_K1723012 BBa_K1723013 BBa_K1723014 BBa_K1723015 BBa_K1723016 BBa_K1723017 BBa_K1723018 BBa_K1723019 BBa_K1723020 |
Silver medal
| Criterion | Description | Link |
|---|---|---|
| Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected. | For E. coli we created new BioBricks and we were able to test them experimentally (cf. our Results page). As for S. cerevisiae these new BioBricks consists on dCas9 fused with a RNA polymerase subunit and synthetic gRNAs. In addition we biobriked a promoter from Bikard et al. and a new entirely synthetic version of it containing others gRNA targeted sites. | BBa_K1723000 BBa_K1723005 BBa_K1723002 BBa_K1723003 BBa_K1723004 BBa_K1723006 BBa_K1723007 BBa_K1723008 |
| Submit this new part to the iGEM Parts Registry. | All the parts created and experimentally validated in E. coli are submitted to the iGEM Parts Registry. Note that also S. cerevisiae BioBricks (presented as a Bronze Medal requirement) are submitted to the iGEM Parts Registry. | BBa_K1723000 BBa_K1723005 BBa_K1723002 BBa_K1723003 BBa_K1723004 BBa_K1723006 BBa_K1723007 BBa_K1723008 |
| iGEM projects involve important questions beyond the bench. Demonstrate how your team has identified, investigated and addressed one or more of these questions in the context of your project. | Practices |
Gold medal
| Criterion | Description | Link |
|---|---|---|
| Expand on your silver medal Human Practices activity or demonstrate an innovative Human Practices activity that relates to your project. | Practices | |
| Improve the function or characterization of a previously existing BioBrick Part or Device | Bikard et al. used dCas9-\(\omega\) in order to regulate gene expression. Their dCas9 system works with tracRNAs, while our system works with simpler gRNAs. In order to use our dCas9-gRNA system we had to modify Bikard's promoter. In addition, to test new gRNA sequences we created a modified synthetic version of this promoter. | BBa_K1723000 BBa_K1723001 BBa_K1723005 |
| Demonstrate a functional prototype of your project. | Design |
