Difference between revisions of "Team:UNITN-Trento/Test"

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<h2><strong>Where do we waste energy?</strong></h2>
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<h2><strong>InterLab Measurement Study</strong></h2>
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<p> A brief study on energy and the ways to spare it </p>
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<p>What happens when GFP meets different promoters? Are they all the same? Here is what we found out!</p>
 
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<li><p id="interlab_intro_btn" class="button small bstyle1" style="line-height:2em;" onclick="javascript:scrollToID('interlab_intro')"><span class="primary">Introduction</span> <span class="primary">&amp; Achievements</span></p></li> 
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<h3 class="wow fadeInDown">The Interlab Measurement Study</h3>
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<p class="wow fadeInDown">The characterization of standard parts has always been one of the main concerns in Synthetic Biology. For this very same reason, iGEM teams from all around the World were suggested to take part in the biggest measurement study ever conducted and the 2015 UNITN-Trento iGEM team answered the call. The goal of this <span class="i_enph italic">Second International Measurement Interlab Study</span> is to assemble <span class="i_enph">three different devices</span>, each one containing a <span class="i_enph">promoter</span> with a screening plasmid intermediate and collect as many fluorescence data as possible. iGEM teams are free to use any technique to measure their devices as long <span class="i_enph">the obtained data are solid and reproducible</span>.</p> 
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<h4>Clear Data and Protocols</h4>
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<p>We listed in the &rdquo;InterLab Study&rdquo; page:
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<li>All devices measured for this study</li>
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<li>All protocols developed and adopted</li>
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<li>Sequencing data for all measurements devices</li>
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<h4><span>3</span> BioBrick devices</h4>
 
<h4><span>3</span> BioBrick devices</h4>
 
<p>We used the three BioBrick devices listed in the &rdquo;Required Devices&rdquo; section.</p>
 
<p>We used the three BioBrick devices listed in the &rdquo;Required Devices&rdquo; section.</p>
 
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<h4><span> 5</span> techniques </h4>
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<p>We used <span class="i_enph italic">in-vivo</span> and <span class="i_enph italic">in-vitro</span> techniques for measuring RNA and protein levels:
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<li>Plate Reader</li>
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<li>Spectrofluorometer</li>
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<li>Flow Cytometer</li>
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<li>RT-qPCR</li>
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<li>Cell-Free Extract</li>
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<h4> Statistical Reliability </h4>
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<p>We have three biological replicates for each measurement, with positive and negative controls</p>
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<h4>Worksheet and Protocol</h4>
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<p>We completed the InterLab Worksheet and the InterLab Protocol</p>
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<h4>Extra Credit Assignment</h4>
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<p>We did technical replicates for each sample</p>
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Revision as of 01:55, 17 September 2015

InterLab Measurement Study

What happens when GFP meets different promoters? Are they all the same? Here is what we found out!

  • Introduction & Achievements

  • Experimental Design

  • Experiments& Protocols

  • Final Discussion & Results

The Interlab Measurement Study

The characterization of standard parts has always been one of the main concerns in Synthetic Biology. For this very same reason, iGEM teams from all around the World were suggested to take part in the biggest measurement study ever conducted and the 2015 UNITN-Trento iGEM team answered the call. The goal of this Second International Measurement Interlab Study is to assemble three different devices, each one containing a promoter with a screening plasmid intermediate and collect as many fluorescence data as possible. iGEM teams are free to use any technique to measure their devices as long the obtained data are solid and reproducible.

Clear Data and Protocols

We listed in the ”InterLab Study” page:

  • All devices measured for this study
  • All protocols developed and adopted
  • Sequencing data for all measurements devices

3 BioBrick devices

We used the three BioBrick devices listed in the ”Required Devices” section.

5 techniques

We used in-vivo and in-vitro techniques for measuring RNA and protein levels:

  • Plate Reader
  • Spectrofluorometer
  • Flow Cytometer
  • RT-qPCR
  • Cell-Free Extract

Statistical Reliability

We have three biological replicates for each measurement, with positive and negative controls

Worksheet and Protocol

We completed the InterLab Worksheet and the InterLab Protocol

Extra Credit Assignment

We did technical replicates for each sample