Difference between revisions of "Team:Yale/collaborations"

Revision as of 03:07, 17 September 2015

<!DOCTYPE html> Yale iGem 2015: Collaborations

Collaborations

Featuring University of La Verne!

Developing a Guidebook - University of La Verne iGEM

Beginning Research in Non-Model Microbes

Over the past few years, iGEM has increasingly centered around non-model microorganisms—organisms which are less well-characterized and have fewer resources for genetic manipulation than the model E. coli and S. cerevisiae. Despite a prevalence of non-model organisms in iGEM competition projects and the potential impact which these projects may have, few resources exist for teams hoping to initiate research in non-model strains.

The 2015 Yale University iGEM team has collaborated with several other teams working in non-model strains to design a set of considerations for future iGEM teams in order to reduce the barrier to entry into non-model organisms. We synthesized the experiences of other teams into this handout, which has been made available on our team wiki.

ProtoCat - University of Michigan

A Centralized, Validated Protocols Database

Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.

@@ Line 57: / Line 57: @@
      <section class="content__section">
        <h2 id="overview">Collaborations</h2>
-       <h3>Featuring University of La Verne and the University of Michigan!</h3>
+       <h3>Featuring University of La Verne!</h3>
        <div class="page__button"><a href="#guidebook" class="custom__button">Guidebook</a><a href="#protocat" class="custom__button">ProtoCat</a>
        </div>
      </section>
      <section class="content__section__alt">
-       <h2 id="guidebook">Developing a Guidebook - <a href="#" target="_blank" class="uni__link">University of La Verne iGEM</a></h2>
+       <h2 id="guidebook">Developing a Guidebook - <a href="#" target="https://2015.igem.org/Team:LaVerne-Leos" class="uni__link">University of La Verne iGEM</a></h2>
        <h3>Beginning Research in Non-Model Microbes</h3>
-       <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
+       <p>Over the past few years, iGEM has increasingly centered around non-model microorganisms—organisms which are less well-characterized and have fewer resources for genetic manipulation than the model E. coli and S. cerevisiae. Despite a prevalence of non-model organisms in iGEM competition projects and the potential impact which these projects may have, few resources exist for teams hoping to initiate research in non-model strains.</p>
-      <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
+       <p>The 2015 Yale University iGEM team has collaborated with several other teams working in non-model strains to design a set of considerations for future iGEM teams in order to reduce the barrier to entry into non-model organisms. We synthesized the experiences of other teams into this handout, which has been made available on our team wiki. </p>
-       <p>Biofilm formation on surfaces is an issue in the medical field, naval industry, and other areas. We developed an anti-fouling peptide with two modular components: a mussel adhesion protein (MAP) anchor and LL-37, an antimicrobial peptide. MAPs can selectively attach to metal and organic surfaces via L-3,5-dihydroxyphenylalanine (L-DOPA), a nonstandard amino acid that was incorporated using a genetically recoded organism (GRO). Because this peptide is toxic to the GRO in which it is produced, we designed a better controlled inducible system that limits basal expression. This was achieved through a novel T7 riboregulation system that controls expression at both the transcriptional and translational levels.</p>
      </section>
      <section class="content__section">