Difference between revisions of "Team:SPSingapore/Notebook-Week-2"

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<table><tr><td>
 
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<div style = "border-top: 5px solid blue; width:750px; text-align:justify;margin-bottom: 20px; line-height:normal;">
<h3 style ="margin-left:20px;">Week 2 (31/5 - 6/6)</h3>
+
<h3 style ="margin-left:20px;">Week 16 (6/9 - 12/9)</h3>
  
  
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<!----------------------- Entry Start ------------------------>
 
<!----------------------- Entry Start ------------------------>
 
<center>
 
<center>
<table style = "width:700px; border-collapse: collapse;">
+
<table style = "width:750px; border-collapse: collapse;">
  
<tr class = "tablenotebook"><td><p class = "paper">
+
<! ---------DAY ONE ------------------------------->
May 31
+
 
<br><br>
+
<tr class = "tablenotebook"><td><div class = "paper">
<strong>Xin Yi</strong>
+
&#9642; May 31
 
<br><br>
 
<br><br>
&bull; Sent EsaR clone 4 and INv-4 for sequencing.
+
<span class = "blue"> Invasin + Listerolysin</span>
 +
&emsp;&emsp; <span class = "black"> ESA Quorum Sensing</span>
 
<br>
 
<br>
</p>
+
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Xin Yi &#9728;</font>
 +
<br><br>
 +
<div class = "divnbcontent">
 +
<span class = "nbcontent">
 +
Sent EsaR clone 4 and INv-4 for sequencing.
 +
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
  
<tr class = "tablenotebook"><td><p class = "paper">
+
 
June 2
+
<!---------------next day ----------------------------------------->
 +
 
 +
<tr class = "tablenotebook"><td><div class = "paper">
 +
&#9642; June 2
 
<br><br>
 
<br><br>
<strong>Yi Han</strong>
+
<span class = "brown"> Anaerobic Promoter</span>
 +
&emsp;&emsp;
 +
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Xin Yi &#9728;</font>
 
<br><br>
 
<br><br>
&bull; YFP and GFP transformation results - no colonies for YFP<br>
+
<div class = "divnbcontent">
&bull; The GFP transformation repeated with 100ng of plasmid was sucessful.<br>
+
<span class = "nbcontent">
&bull; 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight.
+
YFP and GFP transformation results - no colonies for YFP
<br>
+
</span><br>
</p>
+
<span class = "nbcontent">
 +
The GFP transformation repeated with 100ng of plasmid was sucessful. 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight.
 +
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
  
<tr class = "tablenotebook"><td><p class = "paper">
+
 
June 3
+
<!---------------next day ----------------------------------------->
 +
 
 +
<tr class = "tablenotebook"><td><div class = "paper">
 +
&#9642; June 3
 
<br><br>
 
<br><br>
<strong>Yi Han</strong>
+
<span class = "brown"> Anaerobic Promoter</span>
 +
&emsp;&emsp;
 +
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Yi Han &#9728;</font>
 
<br><br>
 
<br><br>
&bull; Miniprep of gfp plasmids  
+
<div class = "divnbcontent">
<br>
+
<span class = "nbcontent">
&bull; RE digest with EcoRI and RsaI
+
Miniprep of gfp plasmids
</p>
+
</span><br>
 +
<span class = "nbcontent">
 +
RE digest with EcoRI and RsaI
 +
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
  
<tr class = "tablenotebook"><td><p class = "paper">
+
<!---------------next day ----------------------------------------->
June 4
+
 
 +
 
 +
<tr class = "tablenotebook"><td><div class = "paper">
 +
&#9642; June 4
 
<br><br>
 
<br><br>
<strong>Chi Yan</strong>
+
<span class = "brown"> Anaerobic Promoter</span>
 +
&emsp;&emsp;
 +
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Chi Yan &#9728;</font>
 
<br><br>
 
<br><br>
&bull; RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones
+
<div class = "divnbcontent">
<br>
+
<span class = "nbcontent">
</p>
+
RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones
 +
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
  
<tr class = "tablenotebook"><td><p class = "paper">
+
<!---------------next day ----------------------------------------->
June 5
+
 
 +
<tr class = "tablenotebook"><td><div class = "paper">
 +
&#9642; June 5
 
<br><br>
 
<br><br>
<strong>Yan Ting</strong>
+
<span class = "blue"> Invasin + Listerolysin</span>
 +
&emsp;&emsp;<span class = "black"> ESA Quorum Sensing</span>
 +
<br>
 +
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Yan Ting &#9728;</font>
 
<br><br>
 
<br><br>
&bull; Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic
+
<div class = "divnbcontent">
 +
<span class = "nbcontent">
 +
Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic
 +
</span>
 
<br>
 
<br>
&bull; Cell culture &rarr; HEK293 cells revived from freezing down appeared detached.
+
<span class = "nbcontent">
<br>
+
Cell culture-> HEK293 cells revived from freezing down appeared detached.
</p>
+
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
  
<tr class = "tablenotebook"><td><p class = "paper">
+
 
June 6
+
<!---------------next day ----------------------------------------->
 +
 
 +
<tr class = "tablenotebook"><td><div class = "paper">
 +
&#9642; June 5
 
<br><br>
 
<br><br>
<strong>Yi Han & Yan Ting</strong>
+
<span class = "blue"> Invasin + Listerolysin</span>
 +
&emsp;&emsp;<span class = "black"> ESA Quorum Sensing</span>
 +
&emsp;&emsp;<span class = "brown"> Anaerobic Promoter</span>
 +
<br>
 +
<font style = "color:blue;font-weight:bold;font-size:11pt;">&#9728; Yi Han &#9728;</font>
 
<br><br>
 
<br><br>
&bull; Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol
+
<div class = "divnbcontent">
 +
<span class = "nbcontent">
 +
Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol
 +
</span>
 
<br>
 
<br>
&bull; Yan Ting: grew HEK293T cells in T25 glask
+
<span class = "nbcontent">
 +
Yanting: grew HEK293T cells in T25 flask
 +
</span>
 
<br>
 
<br>
&bull; Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.
+
<span class = "nbcontent">
<br><br><br>
+
Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.
</p>
+
</span>
 +
</div>
 +
 
 +
<br><br>
 +
 
 +
</div>
 +
<div style = "border-top: 2px solid turquoise;"></div>
 
</td>
 
</td>
 
</tr>
 
</tr>
 +
 +
<!---------------next day ----------------------------------------->
 +
 +
  
 
</table>
 
</table>

Revision as of 06:54, 17 September 2015


Research Notebook

Week 16 (6/9 - 12/9)

▪ May 31

Invasin + Listerolysin    ESA Quorum Sensing
☀ Xin Yi ☀

Sent EsaR clone 4 and INv-4 for sequencing.


▪ June 2

Anaerobic Promoter    ☀ Xin Yi ☀

YFP and GFP transformation results - no colonies for YFP
The GFP transformation repeated with 100ng of plasmid was sucessful. 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight.


▪ June 3

Anaerobic Promoter    ☀ Yi Han ☀

Miniprep of gfp plasmids
RE digest with EcoRI and RsaI


▪ June 4

Anaerobic Promoter    ☀ Chi Yan ☀

RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones


▪ June 5

Invasin + Listerolysin    ESA Quorum Sensing
☀ Yan Ting ☀

Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic
Cell culture-> HEK293 cells revived from freezing down appeared detached.


▪ June 5

Invasin + Listerolysin    ESA Quorum Sensing    Anaerobic Promoter
☀ Yi Han ☀

Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol
Yanting: grew HEK293T cells in T25 flask
Gel extract to clean up gfp plasmids which loading dye had accidentally been added to.