Difference between revisions of "Team:SPSingapore/Notebook-Week-2"
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+ | .blue{ | ||
+ | color:white;font-weight:bold;font-size:11pt;background-color:blue; | ||
+ | box-shadow: 0 0 0 5px blue; | ||
+ | } | ||
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<table><tr><td> | <table><tr><td> | ||
<div style = "border-top: 5px solid blue; width:750px; text-align:justify;margin-bottom: 20px; line-height:normal;"> | <div style = "border-top: 5px solid blue; width:750px; text-align:justify;margin-bottom: 20px; line-height:normal;"> | ||
− | <h3 style ="margin-left:20px;">Week | + | <h3 style ="margin-left:20px;">Week 16 (6/9 - 12/9)</h3> |
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<!----------------------- Entry Start ------------------------> | <!----------------------- Entry Start ------------------------> | ||
<center> | <center> | ||
− | <table style = "width: | + | <table style = "width:750px; border-collapse: collapse;"> |
− | <tr class = "tablenotebook"><td>< | + | <! ---------DAY ONE -------------------------------> |
− | May 31 | + | |
− | + | <tr class = "tablenotebook"><td><div class = "paper"> | |
− | + | ▪ May 31 | |
<br><br> | <br><br> | ||
− | & | + | <span class = "blue"> Invasin + Listerolysin</span> |
+ |    <span class = "black"> ESA Quorum Sensing</span> | ||
<br> | <br> | ||
− | </ | + | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Xin Yi ☀</font> |
+ | <br><br> | ||
+ | <div class = "divnbcontent"> | ||
+ | <span class = "nbcontent"> | ||
+ | Sent EsaR clone 4 and INv-4 for sequencing. | ||
+ | </span> | ||
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
− | <tr class = "tablenotebook"><td>< | + | |
− | June 2 | + | <!---------------next day -----------------------------------------> |
+ | |||
+ | <tr class = "tablenotebook"><td><div class = "paper"> | ||
+ | ▪ June 2 | ||
<br><br> | <br><br> | ||
− | < | + | <span class = "brown"> Anaerobic Promoter</span> |
+ |    | ||
+ | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Xin Yi ☀</font> | ||
<br><br> | <br><br> | ||
− | + | <div class = "divnbcontent"> | |
− | + | <span class = "nbcontent"> | |
− | + | YFP and GFP transformation results - no colonies for YFP | |
− | <br> | + | </span><br> |
− | </ | + | <span class = "nbcontent"> |
+ | The GFP transformation repeated with 100ng of plasmid was sucessful. 4 colonies of gfp plasmid were inoculated in 3mL LB+amp and grown overnight. | ||
+ | </span> | ||
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
− | <tr class = "tablenotebook"><td>< | + | |
− | June 3 | + | <!---------------next day -----------------------------------------> |
+ | |||
+ | <tr class = "tablenotebook"><td><div class = "paper"> | ||
+ | ▪ June 3 | ||
<br><br> | <br><br> | ||
− | < | + | <span class = "brown"> Anaerobic Promoter</span> |
+ |    | ||
+ | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yi Han ☀</font> | ||
<br><br> | <br><br> | ||
− | + | <div class = "divnbcontent"> | |
− | <br> | + | <span class = "nbcontent"> |
− | + | Miniprep of gfp plasmids | |
− | </ | + | </span><br> |
+ | <span class = "nbcontent"> | ||
+ | RE digest with EcoRI and RsaI | ||
+ | </span> | ||
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
− | <tr class = "tablenotebook"><td>< | + | <!---------------next day -----------------------------------------> |
− | June 4 | + | |
+ | |||
+ | <tr class = "tablenotebook"><td><div class = "paper"> | ||
+ | ▪ June 4 | ||
<br><br> | <br><br> | ||
− | < | + | <span class = "brown"> Anaerobic Promoter</span> |
+ |    | ||
+ | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Chi Yan ☀</font> | ||
<br><br> | <br><br> | ||
− | + | <div class = "divnbcontent"> | |
− | <br> | + | <span class = "nbcontent"> |
− | </ | + | RE digest indicated a very faint smaller band for colonies 2-4, and hence these were likely to be positive clones |
+ | </span> | ||
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
− | <tr class = "tablenotebook"><td>< | + | <!---------------next day -----------------------------------------> |
− | June 5 | + | |
+ | <tr class = "tablenotebook"><td><div class = "paper"> | ||
+ | ▪ June 5 | ||
<br><br> | <br><br> | ||
− | < | + | <span class = "blue"> Invasin + Listerolysin</span> |
+ |   <span class = "black"> ESA Quorum Sensing</span> | ||
+ | <br> | ||
+ | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yan Ting ☀</font> | ||
<br><br> | <br><br> | ||
− | + | <div class = "divnbcontent"> | |
+ | <span class = "nbcontent"> | ||
+ | Inoculated 3mLS of Inv-4 and EsaR-4 plasmid carrying bacteria into 100mL LB+ appropriate Antibiotic | ||
+ | </span> | ||
<br> | <br> | ||
− | + | <span class = "nbcontent"> | |
− | <br> | + | Cell culture-> HEK293 cells revived from freezing down appeared detached. |
− | </ | + | </span> |
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
− | <tr class = "tablenotebook"><td>< | + | |
− | June | + | <!---------------next day -----------------------------------------> |
+ | |||
+ | <tr class = "tablenotebook"><td><div class = "paper"> | ||
+ | ▪ June 5 | ||
<br><br> | <br><br> | ||
− | < | + | <span class = "blue"> Invasin + Listerolysin</span> |
+ |   <span class = "black"> ESA Quorum Sensing</span> | ||
+ |   <span class = "brown"> Anaerobic Promoter</span> | ||
+ | <br> | ||
+ | <font style = "color:blue;font-weight:bold;font-size:11pt;">☀ Yi Han ☀</font> | ||
<br><br> | <br><br> | ||
− | + | <div class = "divnbcontent"> | |
+ | <span class = "nbcontent"> | ||
+ | Storage of bacterial glycerol stocks for Inv-4, EsaR4 in 25% glycerol | ||
+ | </span> | ||
<br> | <br> | ||
− | + | <span class = "nbcontent"> | |
+ | Yanting: grew HEK293T cells in T25 flask | ||
+ | </span> | ||
<br> | <br> | ||
− | + | <span class = "nbcontent"> | |
− | < | + | Gel extract to clean up gfp plasmids which loading dye had accidentally been added to. |
− | </ | + | </span> |
+ | </div> | ||
+ | |||
+ | <br><br> | ||
+ | |||
+ | </div> | ||
+ | <div style = "border-top: 2px solid turquoise;"></div> | ||
</td> | </td> | ||
</tr> | </tr> | ||
+ | |||
+ | <!---------------next day -----------------------------------------> | ||
+ | |||
+ | |||
</table> | </table> |
Revision as of 06:54, 17 September 2015
Research Notebook |
Week 16 (6/9 - 12/9)
|