Difference between revisions of "Team:Technion HS Israel/Notebook"
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</table> | </table> | ||
</div> | </div> | ||
| + | </ul> | ||
| + | </div> | ||
| + | <a name="NanoBio Protocol for gene knockout"><h4>NanoBio Protocol for gene knockout</h4></a> | ||
| + | <!--1/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/5/5e/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p1.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p1 out of 7" style="width:577px; height:899px;" > | ||
| + | <!--2/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/1/14/Team-Technion_HS_Israel_NanoBio_Protocol_for_gene_Knockout_p2.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p2 out of 7" style="width:645px; height:893px;" > | ||
| + | <!--3/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/9/9a/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p3.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p3 out of 7" style="width:613px; height:885px;" > | ||
| + | <!--4/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/c/ce/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p4.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p4 out of 7" style="width:608px; height:886px;" > | ||
| + | <!--5/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/41/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p5.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p5 out of 7" style="width:606px; height:885px;" > | ||
| + | <!--6/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/61/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p6.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p6 out of 7" style="width:607px; height:887px;" > | ||
| + | <!--7/7--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/2/24/Team-Technion_HS_Isreal_NanoBio_Protocol_for_gene_Knockout_p7.PNG" alt="Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p7 out of 7" style="width:627px; height:887px;" > | ||
| + | |||
| + | <a name="Antibiotics preparation"></a><h4>Antibiotics preparation</h4></a> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/e/e8/Team-Technion_HS_Isreal_Antibiotics_preparation_p1.PNG" alt="Team-Technion HS Isreal Technion-Israel-Antibiotics p1 of 1" style="width:606px; height:966px;" > | ||
| + | |||
| + | <a name="Antibiotics preparation"></a><h4>Antibiotics preparation</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/e/e8/Team-Technion_HS_Isreal_Antibiotics_preparation_p1.PNG" alt="Team-Technion HS Isreal Technion-Israel-Antibiotics p1 of 1" style="width:606px; height:966px;" > | ||
| + | |||
| + | <a name="Chemical transformation"></a><h4>Chemical transformation</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/9/9c/Team-Technion_HS_Isreal_Technion-Israel-Chemical_transformation_p1.PNG" alt="Team-Technion HS Isreal Technion-Israel-Chemical_transformation_p1 of 1" style="width:607px; height:965px;" > | ||
| + | |||
| + | <a name="DNA_Kit_Plate"></a><h4>DNA Kit Plate</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/0/07/Team-Technion_HS_Isreal_Technion-Israel-DNA_Kit_Plate.PNG" alt="Team-Technion HS Isreal Technion-Israel-DNA_Kit_Plate_p1 of 1" style="width:691px; height:609px;" > | ||
| + | |||
| + | <a name="Gel_and_PCR_Extraction"></a><h4>Gel and PCR Extraction</h4> | ||
| + | <!--1/4--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/9/94/Team-Technion_HS_Isreal_Technion-Isreal-Gel_and_PCR_Extraction_p1.PNG" alt="Team-Technion HS Isreal Technion-Israel-Gel_and_PCR_Extraction p1 of 4" style="width:459px; height:646px;" > | ||
| + | |||
| + | |||
| + | <!--2/4--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/68/Team-Technion_HS_Isreal_Technion-Isreal-Gel_and_PCR_Extraction_p2.PNG" alt="Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p2 of 4" style="width:456px; height:642px;" > | ||
| + | |||
| + | |||
| + | <!--3/4--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/4a/Team-Technion_HS_Isreal_Technion-Isreal-Gel_and_PCR_Extraction_p3.PNG" alt="Team-Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p3 of 4" style="width:457px; height:644px;" > | ||
| + | |||
| + | <!--4/4--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/6/61/Team-Technion_HS_Isreal_Technion-Isreal-Gel_and_PCR_Extraction_p4.PNG" alt="Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p4 of 4" style="width:455px; height:645px;" > | ||
| + | |||
| + | <a name="Gel Prep"></a><h4>Gel Prep</h4> | ||
| + | <!--1+2/2--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/0/0f/Team_Technion_HS_Israel_Technion-Israel-Gel_Prep_p1%262.PNG" alt="TTeam Technion HS Israel Technion-Israel-Gel_Prep_p1&2 of 2" style="width:914px; height:646px;" > | ||
| + | |||
| + | <a name="Glycerol stock"></a><h4>Glycerol stock</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/48/Team_Technion_HS_Israel_Technion-Israel-Glycerol_stock_p1.PNG" alt="Team Technion HS Israel Technion-Israel-Glycerol_stock_p1 of 1" style="width:616px; height:647px;" > | ||
| + | |||
| + | <a name="LB SOB SOC BA"></a><h4>LB SOB SOC BA</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/4/41/Team-Technion_HS_Israel_Technion-Israel-LB_SOB_SOC_BA_protocol_p1.PNG" alt="Team-Technion HS Israel Technion-Israel-LB_SOB_SOC_BA_protocol_p1 of 1" style="width:691px; height:614px;" > | ||
| + | |||
| + | <a name="Ligation"></a><h4>Ligation</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/e/ee/Team-Technion_HS_Israel_Technion-Israel-Ligation_p1.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-Ligation_p1 of 1" style="width:611px; height:653px;" > | ||
| + | |||
| + | <a name="manual NEB gibson assembly"></a><h4>manual NEB (gibson assembly) </h4> | ||
| + | <!--is this ok? is it copyright or something?--!> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/f/f8/Team-Technion_HS_Israel_Technion-Israel-manual_NEB_%28gibson_assembly%29_coverpage.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-anual_NEB_(gibson_assembly)_coverpage" style="width:407px; height:646px;" > | ||
| + | |||
| + | |||
| + | <a name="MixandGo"></a><h4>MixandGo</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/a/ad/Team-Technion_HS_Israel_Technion-Israel-MixandGo_coverpage.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-MixandGo_coverpage" style="width:502px; height:648px;" > | ||
| + | |||
| + | <a name="PCR"></a><h4>PCR</h4> | ||
| + | <!--1+2/3--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/c/c7/Team-Technion_HS_Israel_Technion-Israel-PCR_p1%262.PNG" alt="Team-Team-Technion HS Israel /protocols/Technion-Israel-PCR_p1&2 of 3" style="width:915px; height:645px;" > | ||
| + | |||
| + | <!--3/3--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/2/22/Team-Technion_HS_Israel_Technion-Israel-PCR_p3.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-PCR_p3 of 3" style="width:458px; height:645px;" > | ||
| + | |||
| + | <a name="Phosphorylation and blunt ligation"></a><h4>Phosphorylation and blunt ligation</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/d/d8/Team-Technion_HS_Israel_Technion-Israel-Phosphorylation_%28PNK%29_and_blunt_ligation_p1.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-Phosphorylation_(PNK)_and_blunt_ligation p1 of 1" style="width:618px; height:650px;" > | ||
| + | |||
| + | <a name="Presto Mini Plasmid Kit Protocol"></a><h4>Presto Mini Plasmid Kit Protocol</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/3/35/Team-Technion_HS_Israel_Technion-Israel-Presto_Mini_Plasmid_Kit_Protocol_p1.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-Presto_Mini_Plasmid_Kit_Protocol_p1 of 1" style="width:615px; height:650px;" > | ||
| + | |||
| + | <a name="Restriction enzyme"></a><h4>Restriction enzyme</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/9/98/Team-Technion_HS_Israel_Technion-Israel-Restriction_enzyme_p1.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-Restriction enzyme p1 of 1" style="width:615px; height:632px;" > | ||
| + | |||
| + | <a name="Transformation to 3300 LG using electroporation"></a><h4>Transformation to 3300 LG using electroporation</h4> | ||
| + | <!--1/1--!> | ||
| + | <img src="https://static.igem.org/mediawiki/2015/7/74/Team-Technion_HS_Israel_Technion-Israel-Transformation_to_3300_LG_using_electroporation_p1.PNG" alt="Team-Technion HS Israel /protocols/Technion-Israel-Transformation_to_3300_LG_using_electroporation_p1 of 1" style="width:617px; height:624px;" > | ||
Revision as of 07:23, 17 September 2015
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Home
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Modelling
We take our math seriously
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Software
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Team
We are a bunch of nerdy nerds
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Practices
Notebook
Here is a summery of our notebook.
| Week | Design | Cloning | Experiment | Finish new BioBrick |
|---|---|---|---|---|
| Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid . Gblock :RBS- Aiia with a Gibson overlabs. |
we got j09855 part from the distribution kit. | - | - |
| June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa_K1767001 |
| Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. PCR to get the k1767001 plasmid without LuxR . |
- | BBa_K1767002 BBa_K1767004 |
| Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa_K1767003 BBa_K1767005 |
| Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
| Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
| Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa_K1767009 BBa_K1767010 |
| Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |
NanoBio Protocol for gene knockout
Antibiotics preparation
Antibiotics preparation
Chemical transformation
DNA Kit Plate
Gel and PCR Extraction
Gel Prep
Glycerol stock
LB SOB SOC BA
Ligation
manual NEB (gibson assembly)
MixandGo
PCR
Phosphorylation and blunt ligation
Presto Mini Plasmid Kit Protocol
Restriction enzyme
Transformation to 3300 LG using electroporation
