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Revision as of 07:26, 17 September 2015

Technion 2015 HS Team's Wiki

Notebook

Here is a summery of our notebook.

Week Design Cloning Experiment Finish new BioBrick
Jul 5th to Jul 9th (2015) Primers : OGEM 1 and 2 to open j09855 plasmid .

Gblock :RBS- Aiia with a Gibson overlabs.		 we got j09855 part from the distribution kit. - -
June 12th to July 16th (2015) Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. PCR to open the j09855 plasmid.Gibson to add Aiia . - BBa_K1767001
Aug. 2nd to Aug. 6th (2015) - We got p0440 part from the distribution kit.

We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes.

PCR to get the k1767001 plasmid without LuxR .		 - BBa_K1767002

BBa_K1767004
Aug. 9th to Aug. 13th (2015) Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) We got I13521 part from the distribution kit.
We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes.
Colony PCR to check if we have LuxR in the plasmid.		 - BBa_K1767003
BBa_K1767005
Aug. 16th to Aug. 20th (2015) - - AHL experiment #1 -
Aug. 23rd to Aug. 27th (2015) Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). We got I13003 part from the distribution kit. AHL experiment #2 -
Aug. 30th to Sept. 9th (2015) Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.
Colony PCR to check if we have YFP in the plasmid.		 - BBa_K1767009
BBa_K1767010
Sept. 6th to Sept. 11th (2015) - - AHL experiment # 3 -

NanoBio Protocol for gene knockout

 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p1 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p2 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p3 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p4 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p5 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p6 out of 7 Team:Technion_HS_Isreal/Protocols/NanoBio_Protocol_for_gene_Knockout/NanoBio_Protocol_for_gene_Knockout p7 out of 7

Antibiotics preparation

Team-Technion HS Isreal Technion-Israel-Antibiotics p1 of 1

Antibiotics preparation

Team-Technion HS Isreal Technion-Israel-Antibiotics p1 of 1

Chemical transformation

Team-Technion HS Isreal Technion-Israel-Chemical_transformation_p1 of 1

DNA Kit Plate

Team-Technion HS Isreal Technion-Israel-DNA_Kit_Plate_p1 of 1

Gel and PCR Extraction

Team-Technion HS Isreal Technion-Israel-Gel_and_PCR_Extraction p1 of 4 Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p2 of 4 Team-Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p3 of 4 Team-Technion HS Isreal Technion-Isreal-Gel_and_PCR_Extraction_p4 of 4

Gel Prep

TTeam Technion HS Israel Technion-Israel-Gel_Prep_p1&2 of 2

Glycerol stock

Team Technion HS Israel Technion-Israel-Glycerol_stock_p1 of 1

LB SOB SOC BA

Team-Technion HS Israel Technion-Israel-LB_SOB_SOC_BA_protocol_p1 of 1

Ligation

Team-Technion HS Israel /protocols/Technion-Israel-Ligation_p1 of 1

manual NEB (gibson assembly)

Team-Technion HS Israel /protocols/Technion-Israel-anual_NEB_(gibson_assembly)_coverpage

MixandGo

Team-Technion HS Israel /protocols/Technion-Israel-MixandGo_coverpage

PCR

Team-Team-Technion HS Israel /protocols/Technion-Israel-PCR_p1&2 of 3 Team-Technion HS Israel /protocols/Technion-Israel-PCR_p3 of 3

Phosphorylation and blunt ligation

Team-Technion HS Israel /protocols/Technion-Israel-Phosphorylation_(PNK)_and_blunt_ligation p1 of 1

Presto Mini Plasmid Kit Protocol

Team-Technion HS Israel /protocols/Technion-Israel-Presto_Mini_Plasmid_Kit_Protocol_p1 of 1

Restriction enzyme

Team-Technion HS Israel /protocols/Technion-Israel-Restriction enzyme p1 of 1

Transformation to 3300 LG using electroporation

Team-Technion HS Israel /protocols/Technion-Israel-Transformation_to_3300_LG_using_electroporation_p1 of 1