Difference between revisions of "Team:EPF Lausanne/Practices"

Line 87: Line 87:
 
<h4>Lab immersion</h4>
 
<h4>Lab immersion</h4>
 
<p>Even the most renowned scientist started by pipetting water, this is why we prepared a few activities in the lab to let the students see how it is to work in the world of synthetic biology.[insert activities]</p>
 
<p>Even the most renowned scientist started by pipetting water, this is why we prepared a few activities in the lab to let the students see how it is to work in the world of synthetic biology.[insert activities]</p>
 +
<div class="row">
 +
<div class="col-lg-4"><img src="https://static.igem.org/mediawiki/2015/d/df/EPF_Lausanne_Delphine_Laurie_Unicorn.jpg" alt="Unicorn"></div>
 +
<div class="col-lg-4"><img src="https://static.igem.org/mediawiki/2015/8/8c/EPF_Lausanne_Léa_Francesca_MonaLisa.jpg" alt="Mona Lisa"></div>
 +
<div class="col-lg-4"><img src="https://static.igem.org/mediawiki/2015/c/c0/EPF_Lausanne_Lili_Jojo_Sun.jpg" alt="Sun"></div>
 +
</div>
 
<h4>Ethics debate</h4>
 
<h4>Ethics debate</h4>
 
<p>The future use of novel technologies is a matter that every scientist with integrity has to think about. To make the students reflect about actions and consequences we prepared an ethics debate during which we gave the students a role with a specific opinion. The goal was to make them think of opinions that diverge from their own and to make the activity more fun with a little drama. At the end of the debate, we took some time to discuss the activity withouth the roles. We were pleased to see that what we had opened their eyes on different aspects concerning the responsibilities linked to research in the field of synthetic biology. [maybe talk about swiss votation system?]</p>
 
<p>The future use of novel technologies is a matter that every scientist with integrity has to think about. To make the students reflect about actions and consequences we prepared an ethics debate during which we gave the students a role with a specific opinion. The goal was to make them think of opinions that diverge from their own and to make the activity more fun with a little drama. At the end of the debate, we took some time to discuss the activity withouth the roles. We were pleased to see that what we had opened their eyes on different aspects concerning the responsibilities linked to research in the field of synthetic biology. [maybe talk about swiss votation system?]</p>
Line 174: Line 179:
 
<p>On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. We submitted them our survey and took the opportunity to answer questions about synthetic biology, iGEM and our project. About fifty people stopped by, talking with them was very interesting and open-minding.</p>
 
<p>On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. We submitted them our survey and took the opportunity to answer questions about synthetic biology, iGEM and our project. About fifty people stopped by, talking with them was very interesting and open-minding.</p>
 
   
 
   
−
<p>To begin with our investigations of the following matters : communication, interaction and responsibility in scientific research, we decided to ask the general public about it. On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our dear town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. This allowed us to better spot the expectations that people have towards the scientific community as well as opening our minds to views we are not used to meet as scientific students. It was also an opportunity to introduce synthetic biology to those who did not know about it. Here is a summary of the survey and an analysis of the most interesting results.</p>
+
<p>To begin with our investigations of the following matters : communication, interaction and responsibility in scientific research, we decided to ask the general public about it. On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our dear town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. This allowed us to better spot the expectations that people have towards the scientific community as well as opening our minds to views we are not used to meet as scientific students. It was also an opportunity to introduce synthetic biology to those who did not know about it. Here is a summary of the survey and an analysis of the most interesting results.</p>
  
−
<p>We began by asking the subject whether he/she had ever heard about synthetic biology, and if the answer is no, we provided him/her with the Wikipedia definition: “Synthetic biology is a scientific field combining biology and engineering in order to  design and build (synthesize) new biologic systems and units”. Note that the definition does not mention the word GMO, as this has its importance later on. It was clear to us that synthetic biology still is not a popular expression, as most people had never heard about it.  
+
<p>We began by asking the subject whether he/she had ever heard about synthetic biology, and if the answer is no, we provided him/her with the Wikipedia definition: “Synthetic biology is a scientific field combining biology and engineering in order to  design and build (synthesize) new biologic systems and units”. Note that the definition does not mention the word GMO, as this has its importance later on. It was clear to us that synthetic biology still is not a popular expression, as most people had never heard about it.</p>
−
</p>
+
 
<img class="img-responsive" src="https://static.igem.org/mediawiki/2015/3/3d/EPF_Lausanne_HP_Gauche_1.png" alt="" />
 
<img class="img-responsive" src="https://static.igem.org/mediawiki/2015/3/3d/EPF_Lausanne_HP_Gauche_1.png" alt="" />
 
<p>We then asked the subject whether this definition gave him/her a rather positive or negative feeling about synthetic biology. The answers were enthusiastic, with a vast majority of “rather positive”.</p>
 
<p>We then asked the subject whether this definition gave him/her a rather positive or negative feeling about synthetic biology. The answers were enthusiastic, with a vast majority of “rather positive”.</p>

Revision as of 09:31, 17 September 2015

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

Practices

Main article

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Voluptas nemo et a inventore vero itaque, pariatur impedit perspiciatis reprehenderit. Ipsum cumque aliquid accusantium quae similique, eum esse culpa, officiis! Reiciendis dolorem, nostrum eligendi aliquid. Cupiditate maiores velit praesentium eos adipisci similique, accusantium explicabo, dolores error! Tempore perferendis illo inventore placeat quia velit sunt! Odio maxime, adipisci quis sapiente omnis porro.

  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
  • Nom prénom Profession
HP_Chairs

Titre (peux change la forme facilement)

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Maiores dicta quisquam quibusdam cumque magnam voluptas repellat inventore nisi quia ad, ipsum, consequatur odit, iusto non blanditiis animi placeat excepturi pariatur. Accusamus minus neque soluta maiores ratione, dolore quae animi vitae ex consequatur, eaque dolorum itaque esse velit atque veniam corrupti repellat quasi non commodi est, ad obcaecati earum omnis? Veritatis eligendi, adipisci minima magnam molestias nesciunt ducimus amet dicta sequi eum esse eos. Ipsam sed at nam voluptate. Accusantium officia voluptate minus facilis ullam ipsum soluta iste natus iusto aliquid velit harum, explicabo placeat consequuntur voluptatum sequi eum voluptas maiores vero culpa rerum quas libero voluptatem blanditiis. Nihil minus, ad voluptatibus explicabo rem, harum iure deserunt aliquam velit laboriosam nesciunt nam dolorum et! Iure iusto aliquid quam distinctio quod dolores hic autem quidem laboriosam tempore ducimus veritatis corporis rem dicta beatae explicabo ut, impedit consequatur blanditiis ratione magnam aspernatur sapiente perferendis laudantium perspiciatis. Alias suscipit, harum voluptate voluptatum dolorum assumenda labore laborum, quos ipsam reprehenderit amet quod, praesentium eius, error facere numquam reiciendis! Eligendi aspernatur ab reiciendis, consequatur tempore deserunt rerum nemo laborum nesciunt aperiam, praesentium doloremque quo. Autem explicabo aliquam a hic magni accusamus assumenda nobis, molestias similique, soluta.

Pour mettre du texte en italique met juste une borne i

Sous titre

Lorem ipsum dolor sit amet, consectetur adipisicing elit. Molestiae maxime porro obcaecati, adipisci libero autem nisi rem quaerat at perferendis, nam laboriosam fuga consectetur voluptatum minima! Facilis soluta, dignissimos eveniet distinctio minus in quia odio incidunt nobis earum doloremque repellendus maxime, excepturi consequuntur officiis ducimus. Quia reiciendis accusamus, amet vitae blanditiis aliquid, temporibus cupiditate eaque non et commodi tempora? Incidunt impedit autem repellat enim, corrupti qui, nobis minus, maxime, consectetur obcaecati earum praesentium iste minima! Molestias expedita, similique quos omnis doloribus qui explicabo consequatur, quo, vitae dignissimos quaerat molestiae minus voluptatem quasi debitis dolore repellendus sit, accusamus dolor soluta quas. Nemo iste ab aut sed natus enim odit, commodi, dignissimos placeat cumque! Consequuntur assumenda cumque ad debitis sapiente voluptatum architecto fugiat excepturi facilis! Distinctio dolorem incidunt atque assumenda deserunt eaque nisi sed nam facilis beatae at vel, exercitationem non voluptatum qui reiciendis nobis impedit voluptate quia repellat. Voluptatibus officiis, repellat quas in culpa aperiam architecto animi nihil qui. Excepturi optio, accusantium fugit ipsum, molestiae quam nobis blanditiis eum numquam sint harum omnis eius quidem sapiente doloribus soluta! Fuga, vero quis at facilis animi unde. Tempora laborum voluptatum, impedit illo! Nesciunt, incidunt explicabo. Non fugit voluptatum, impedit sed dolorum soluta tempora?

Highschool students' visit

Start of the day

Since iGEM is encouraging young students to get into the lab and engage in innovative ideas using Synthetic Biology, our aim for this day was to get this mindset across to high school students. In order to do so, we contacted three different schools in our region. The Gymnase d’Yverdon, Collège de Candolle and Gymnase du Bugnon keenly sent us students interested in biology and eager to find out about synthetic biology.

In fact, we found out that, even though the students had advanced biology courses, the material taught does not include synthetic biology. Throughout the day, we did our best to show these students a different side of the field of biology and convince them that we can hack life!

Group Photo
Cyril Presentation

Presentation

We welcomed the students with a presentation that consisted of three parts. The first one was about iGEM, the modalities and principles of the competition, the number of participating teams, the contribution of each team, etc. The second part was about Synthetic Biology. After introducing them to common lab techniques and procedures, we talked about our project, Bio LOGIC.

After the presentation, we seperated them into three groups for three different activities.

mini_iGEM
mini_iGEM
mini_iGEM

Mini iGEM

As we wanted to give them an overview of how the competition works, we made them imagine iGEM projects. In this activity, small groups of students worked together to create iGEM projects and then explained them to the other groups. They were asked to think about feasibility, usefulness, safety and ethics. During the brainstorming, each group was coached by one of our team members. We introduced the concepts of kill switch, biobricks, motility, transcription pathways, transporters and bio-informatics to them. The ideas that they shared with us were surprisingly smart and imaginative for students who had received little technical and background information on the matter. Some teams even brought up and discussed projects that had already been realised in previous iGEM competitions.

Lab immersion

Even the most renowned scientist started by pipetting water, this is why we prepared a few activities in the lab to let the students see how it is to work in the world of synthetic biology.[insert activities]

Unicorn
Mona Lisa
Sun

Ethics debate

The future use of novel technologies is a matter that every scientist with integrity has to think about. To make the students reflect about actions and consequences we prepared an ethics debate during which we gave the students a role with a specific opinion. The goal was to make them think of opinions that diverge from their own and to make the activity more fun with a little drama. At the end of the debate, we took some time to discuss the activity withouth the roles. We were pleased to see that what we had opened their eyes on different aspects concerning the responsibilities linked to research in the field of synthetic biology. [maybe talk about swiss votation system?]

mini_iGEM
mini_iGEM
mini_iGEM

Mini iGEM projects

Name Description
Famousse Famousse ("Mousse" means foam in french) is a gun that sprays an isolating material to fill walls. The fluid consists of bacterias that produce foam once in contact with oxygen and of yeasts that make CO2 holes in the foam. After a while, the foam solidifies and bacterias and yeasts die by apoptosis: the isolating material is finished.
RIDG pill The RIDG pill (Régulateur intelligent Des Graisses or Intelligent Fat Regulator, IFR pill) is made out of bacteria that detect, once in the intestine, if the person suffers of obesity or anorexia thanks to hormone receptors. In case of obesity, the bacteria would respond to that input by secreting proteins that block lipid transporters in epithelial cells. In case of anorexia, the bacteria would secrete hormones that would make epithelial cells create more lipid transporters.
PestiBac Bacteria substituting pesticides. These bacteria live on the leaves of the plants and secrete repelling substances. These bacteria have sensor in order to die if not in contact with the target plant or if they came in contact with animal's saliva. In addition they are modified to have a shorter lifetime, in order to limit population growth.
Luciolight Luciolight is a Street Lamp made out of bacteria that copy biological circuit of fireflies.
Bacteriolavage Bacteriolavage is a bacterial washing system to clean clothes with less water waste. Bacteria synthesize enzymes that degrade lipids and sugars in smaller particles that are more water-soluble, so that less water is consumed. Students used the concept of magnetic bacteria developed by the 2014 iGEM Berlin team to efficiently remove bacterias from clothes once the wash was finished.
Killpilus Killpilus is a permanent hair removal cream. Bacteria contained in the preparation kill hair follicle stem cells. The team searched the internet for receptors specifically expressed in those stem cells to avoid off targets. For safety reasons, bacteria would commit suicide by apoptosis after one hour.
ConchitaColi ConchitaColi cells digest dust and produce scented molecules. After the washing is finished, bacterias move towards a luminous bin where they die by apoptosis.
Healing+ "Healing+" is a system to repair wounds. Mammalian cells circulating in the blood detect intrusion of pathogens and respond by mobilisation of collagen and platelets in wounds.
easyFer "easyFer" ("easyIron") is a system that uses an iron detecting biosensor to simplify the iron detection in the blood.

Public survey in Lausanne


On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. We submitted them our survey and took the opportunity to answer questions about synthetic biology, iGEM and our project. About fifty people stopped by, talking with them was very interesting and open-minding.

To begin with our investigations of the following matters : communication, interaction and responsibility in scientific research, we decided to ask the general public about it. On saturday the 22nd of August from 9 am to 4 pm, we held a stand in Rue Haldimand in our dear town Lausanne. Passerby came and went from and to the market and many of them were intrigued by our poster asking “What is synthetic biology ? Give us your opinion !”. This allowed us to better spot the expectations that people have towards the scientific community as well as opening our minds to views we are not used to meet as scientific students. It was also an opportunity to introduce synthetic biology to those who did not know about it. Here is a summary of the survey and an analysis of the most interesting results.

We began by asking the subject whether he/she had ever heard about synthetic biology, and if the answer is no, we provided him/her with the Wikipedia definition: “Synthetic biology is a scientific field combining biology and engineering in order to design and build (synthesize) new biologic systems and units”. Note that the definition does not mention the word GMO, as this has its importance later on. It was clear to us that synthetic biology still is not a popular expression, as most people had never heard about it.

We then asked the subject whether this definition gave him/her a rather positive or negative feeling about synthetic biology. The answers were enthusiastic, with a vast majority of “rather positive”.

The interesting thing is that after several questions which content we will analyze later, our last question was: “What is your opinion on GMOs (or Genetically Modified Organisms)?”, to which we mostly got “Rather negative” as an answer.



We found it interesting to compare the results of these two diagrams, as they perfectly reflect the misconception people have of the word GMO. We discussed these results with the experts we interviewed, you can read about it in our main article.

The next thing we wanted to know was whether the subject felt a good enough interaction with the scientific world. The answers we got show pretty clearly that it is not the case.

We also wanted to know who, according to the general public, is really responsible for the outcomes of scientific research, and how the subsequent technologies are used. The subject could choose several answers.


We were quite surprised by these results, as in Switzerland, direct democracy and referendums allow the whole population to vote on very specific questions, scientific topics included. And research in synthetic biology could have come to an end if in 1998, the federal popular initiative “for the protection of life and the environment against gene manipulations (initiative for genetic protection)” had been accepted, indeed: production, acquisition and handing-over of any genetically modified animal would have been prohibited. To us, seeing the people delegate the responsibility to researchers is the consequence of a lack of interaction. The subject of interaction is further explored in our main article.

Next, we quickly described the purpose of the iGEM competition, and we exposed the main idea behind our project (building independent transistors in cells). We then introduced some examples of organisms that could be designed using our project, or synthetic biology in general. Some were past iGEM projects, some we imagined ourselves, and they covered a lot of different domains suck as energy, agriculture, medicine and information processing.


The only organism that gathered a vast majority of negative answers was the “Wheat strain capable of adapting to climatic conditions”. To us this is also linked to the associations between GMOs and agriculture.

In order to better understand what exactly was worrying the general public about the usage of synthetic biology, we asked the subject to pick his/her main concerns in the following list: ethics, environment, social, national security, religion and health. Several answers were allowed. The most chosen answer was “environment”. Again, we can observe this association between synthetic biology and environment. “Ethics” only differs by a few answers.

Also, we included a question coming from the Swiss Institute of Technology in Zürich (ETHZ) which intended to determine what was most important between sensitivity and specificity in cancer diagnosis. The results show mixed feelings, and people often took some time to think before answering.

The themes developed in this analysis are discussed in depth in our main article.

EPFL 2015 iGEM bioLogic Logic Orthogonal gRNA Implemented Circuits

NOT PROOFREAD