Difference between revisions of "Team:Technion HS Israel/Notebook"
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Revision as of 13:16, 17 September 2015
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Notebook
Here is a summery of our notebook.
Week | Design | Cloning | Experiment | Finish new BioBrick |
---|---|---|---|---|
Jul 5th to Jul 9th (2015) | Primers : OGEM 1 and 2 to open j09855 plasmid . Gblock :RBS- Aiia with a Gibson overlabs. |
we got j09855 part from the distribution kit. | - | - |
June 12th to July 16th (2015) | Primers : OGEM 3 and 4 to got the k1767001 plasmid without LuxR , after doing the PCR we will get a linear DNA so we put two enzymes in the overlaps of the primers to close the plasmids. | PCR to open the j09855 plasmid.Gibson to add Aiia . | - | BBa_K1767001 |
Aug. 2nd to Aug. 6th (2015) | - | We got p0440 part from the distribution kit. We built BBa_K1767002 and BBa_K1767004 Constructs by restriction enzymes. PCR to get the k1767001 plasmid without LuxR . |
- | BBa_K1767002 BBa_K1767004 |
Aug. 9th to Aug. 13th (2015) | Primers : OGEM 5 and 6 to check if we succeed in the PCR ( Those primers for colony PCR) | We got I13521 part from the distribution kit. We built BBa_K1767003 and BBa_K1767005 Constructs by restriction enzymes. Colony PCR to check if we have LuxR in the plasmid. | - | BBa_K1767003 BBa_K1767005 |
Aug. 16th to Aug. 20th (2015) | - | - | AHL experiment #1 | - |
Aug. 23rd to Aug. 27th (2015) | Primers : OGEM 7 and 8 to check if we have yfp in the plasmid ( for colony PCR ). | We got I13003 part from the distribution kit. | AHL experiment #2 | - |
Aug. 30th to Sept. 9th (2015) | Primers: OGEM 9 and 10 to replaced the RFP with RFP ( with LVA ) | We built BBa_K1767009 and BBa_K1767010 Constructs by restriction enzymes.Colony PCR to check if we have YFP in the plasmid. | - | BBa_K1767009 BBa_K1767010 |
Sept. 6th to Sept. 11th (2015) | - | - | AHL experiment # 3 | - |
preparation
- 1. LB medium
- 2. Miniprep
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d9/Technion_HS_Israel_pre1_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/9/90/Technion_HS_Israel_pre5_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre6](https://static.igem.org/mediawiki/2015/3/3e/Technion_HS_Israel_pre6_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre7](https://static.igem.org/mediawiki/2015/9/94/Technion_HS_Israel_pre7_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre2](https://static.igem.org/mediawiki/2015/8/81/Technion_HS_Israel_pre2_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre3](https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_pre3_2.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre4](https://static.igem.org/mediawiki/2015/3/31/Technion_HS_Israel_pre4_2.jpg)
July 12th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/64/Technion_HS_Israel_12_7.jpg)
July 13th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/50/Technion_HS_Israel_13_7.jpg)
July 15th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_15_7_2.jpg)
July 20th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/6/6b/Technion_HS_Israel_20_7.jpg)
July 22th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_22_7_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/8/86/Technion_HS_Israel_22_7_2.jpg)
July 29th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/a/a2/Technion_HS_Israel_29_7.jpg)
august 2th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/f6/Technion_HS_Israel_2_8.jpg)
august 3th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/85/Technion_HS_Israel_3_8_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/a/a7/Technion_HS_Israel_3_8_2.jpg)
august 4th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/24/Technion_HS_Israel_4_8_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/c/ca/Technion_HS_Israel_4_8_2.jpg)
august 5th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/b8/Technion_HS_Israel_5_8.jpg)
august 6th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/0/02/Technion_HS_Israel_6_8_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_6_8_2.jpg)
august 9th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/3/36/Technion_HS_Israel_9_8.jpg)
august 10th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/8e/Technion_HS_Israel_10_8_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/7/73/Technion_HS_Israel_10_8_2.jpg)
august 11th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/c/ce/Technion_HS_Israel_11_8.jpg)
august 12th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/22/Technion_HS_Israel_12_8.jpg)
august 13th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/f9/Technion_HS_Israel_13_8.jpg)
august 16th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_16_8.jpg)
august 17th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/25/Technion_HS_Israel_17_8.jpg)
august 18th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/e7/Technion_HS_Israel_18_8.jpg)
august 19th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d0/Technion_HS_Israel_19_8.jpg)
august 20th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_20_8.jpg)
august 23th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/1/12/Technion_HS_Israel_23_8.jpg)
august 24th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/ee/Technion_HS_Israel_24_8.jpg)
august 25th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/52/Technion_HS_Israel_25_8.jpg)
august 26th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/f/fe/Technion_HS_Israel_26_8.jpg)
august 27th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/5/56/Technion_HS_Israel_27_8.jpg)
august 29th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/d/d7/Technion_HS_Israel_29_8.jpg)
august 30th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/b/bb/Technion_HS_Israel_30_8.jpg)
august 31th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/1/11/Technion_HS_Israel_31_8.jpg)
september 1th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/e/e6/Technion_HS_Israel_1_9.jpg)
september 2th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/c/c7/Technion_HS_Israel_2_9.jpg)
september 3th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/84/Technion_HS_Israel_3_9.jpg)
september 5th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/7/71/Technion_HS_Israel_5_9.jpg)
september 6th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/8/8a/Technion_HS_Israel_6_9_1.jpg)
![https://2015.igem.org/File:Technion_HS_Israel_pre5](https://static.igem.org/mediawiki/2015/5/5b/Technion_HS_Israel_6_9_2.jpg)
september 7th
![Technion_HS_Israel_pre1](https://static.igem.org/mediawiki/2015/2/23/Technion_HS_Israel_7_9.jpg)