Difference between revisions of "Team:Stockholm/Protocols"
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<h2>Protocols</h2> | <h2>Protocols</h2> | ||
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<a href="https://static.igem.org/mediawiki/2015/f/fb/STHLM_WB_protocol.pdf" >Western blot </a> | <a href="https://static.igem.org/mediawiki/2015/f/fb/STHLM_WB_protocol.pdf" >Western blot </a> | ||
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<a href="https://static.igem.org/mediawiki/2015/c/cc/STHLM_Transformation_protocol.pdf" >Transformation </a> | <a href="https://static.igem.org/mediawiki/2015/c/cc/STHLM_Transformation_protocol.pdf" >Transformation </a> | ||
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<a href="https://static.igem.org/mediawiki/2015/8/84/STHLM_Colony_PCR_protocol.pdf" >Colony PCR</a> | <a href="https://static.igem.org/mediawiki/2015/8/84/STHLM_Colony_PCR_protocol.pdf" >Colony PCR</a> | ||
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<a href="https://static.igem.org/mediawiki/2015/9/91/STHLM_Osmolarity_protocol.pdf" >Osmolarity</a> | <a href="https://static.igem.org/mediawiki/2015/9/91/STHLM_Osmolarity_protocol.pdf" >Osmolarity</a> | ||
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<a href="https://static.igem.org/mediawiki/2015/1/12/STHLM_SiteDirected_mut_protocol.pdf" >Site-directed mutagenesis</a> | <a href="https://static.igem.org/mediawiki/2015/1/12/STHLM_SiteDirected_mut_protocol.pdf" >Site-directed mutagenesis</a> | ||
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<a href="https://static.igem.org/mediawiki/2015/7/75/STHLM_AHL_Osmolarity_protocol.pdf" >AHL osmolarity dependence</a> | <a href="https://static.igem.org/mediawiki/2015/7/75/STHLM_AHL_Osmolarity_protocol.pdf" >AHL osmolarity dependence</a> | ||
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<a href="https://static.igem.org/mediawiki/2015/0/08/STHLM_Cloning_protocol.pdf" >Traditional cloning (Restriction cloning)</a> | <a href="https://static.igem.org/mediawiki/2015/0/08/STHLM_Cloning_protocol.pdf" >Traditional cloning (Restriction cloning)</a> | ||
<br> | <br> | ||
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Revision as of 17:14, 17 September 2015
Protocols
Western blot
Membrane stripping
Spheroplast
FACS
Affinity separation (unfinished)
Dot blot
ELISA
Alternative osmolarity
Overlap PCR
Transformation
Colony PCR
Osmolarity
Site-directed mutagenesis
AHL osmolarity dependence
3A assembly
Chemically competent cells
Overnight cultures and restriction analysis
Traditional cloning (Restriction cloning)