Difference between revisions of "Team:Technion HS Israel/Modelling"

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<img src="https://static.igem.org/mediawiki/2015/7/70/Technion_HS_2015_reactions.png" style="width:80%">
 
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<ol style="list-style-type: lower-roman">
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<li>AHL self-degradation</li>
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<li>Diffusion of AHL</li>
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<li>AHL degradation by aIIa</li>
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<li>Pairing of AHL and LuxR into AHL-LuxR complex</li>
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<li>Degradation of the AHL-LuxR complex into its components</li>
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<li>Pairing of 2 AHL-LuxR complexes into (AHL-LuxR)2</li>
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<li>Degradation of (AHL-LuxR)2 into its components</li>
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<li>(AHL-LuxR)2 binds to the pLuxR promoter</li>
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<li>(AHL-LuxR)2 unbinds from the pLuxR promoter</li>
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<li>Transcription of RNATRLV by pLuxR promoter without the complex</li>
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<li>Transcription of RNATRLV by pLuxR promoter with the complex</li>
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<li>Translation of TRLV from RNATRLV</li>
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<li>TRLV self-degradation</li>
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<li>TRLV binds to the pTetR repressor</li>
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<li>TRLV unbinds from the pLuxR repressor</li>
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<li>Transcription of RNAccdB by ptetR promoter without the TRLV</li>
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<li>Transcription of RNAccdB by ptetR promoter with the TRLV</li>
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<li>Translation of ccdB from RNAccdB</li>
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<li>ccdB self-degradation</li>
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<li>producing of the wanted protein x by the pCONST promoter</li>
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<li>producing of LuxR by the pCONST promoter</li>
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<li>producing of aIIa by the pCONST promoter</li>
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<li>plasmid loss</li>
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<li></li></ol>
 
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Revision as of 21:48, 17 September 2015

Technion 2015 HS Team's Wiki

We're working on it

  1. AHL self-degradation
  2. Diffusion of AHL
  3. AHL degradation by aIIa
  4. Pairing of AHL and LuxR into AHL-LuxR complex
  5. Degradation of the AHL-LuxR complex into its components
  6. Pairing of 2 AHL-LuxR complexes into (AHL-LuxR)2
  7. Degradation of (AHL-LuxR)2 into its components
  8. (AHL-LuxR)2 binds to the pLuxR promoter
  9. (AHL-LuxR)2 unbinds from the pLuxR promoter
  10. Transcription of RNATRLV by pLuxR promoter without the complex
  11. Transcription of RNATRLV by pLuxR promoter with the complex
  12. Translation of TRLV from RNATRLV
  13. TRLV self-degradation
  14. TRLV binds to the pTetR repressor
  15. TRLV unbinds from the pLuxR repressor
  16. Transcription of RNAccdB by ptetR promoter without the TRLV
  17. Transcription of RNAccdB by ptetR promoter with the TRLV
  18. Translation of ccdB from RNAccdB
  19. ccdB self-degradation
  20. producing of the wanted protein x by the pCONST promoter
  21. producing of LuxR by the pCONST promoter
  22. producing of aIIa by the pCONST promoter
  23. plasmid loss