Difference between revisions of "Team:FAU Erlangen/Tour27"

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<p>Vlady: Text</p>
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<h1>Two strains </h1>
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In our study we planned to work with different loci as well as different strains to test under as many circumstances
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as possible. Sadly due to time conflicts we were only able to test with the strain K699.
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<h1>K699</h1>
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This a derivative of strain W303, we used for our transformation.
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Genotype: MATa, ade2-1, trp1-1, can1-100, leu2-3, 112, his3-11,15, ura3, GAL, psi+
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<h1>4196</h1>
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This strain is a derivate of the yeast strain BY4742, which carries an intact copy of the canamycin-gene which can be disrupted by homologous recombination. This was planned to be a second locus of interest next to trp.
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Genotype: BY4742; MATalpha; his3&Delta;1, leu2&Delta0, ura3&Delta0
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Revision as of 22:09, 17 September 2015

Two strains

In our study we planned to work with different loci as well as different strains to test under as many circumstances as possible. Sadly due to time conflicts we were only able to test with the strain K699.

K699

This a derivative of strain W303, we used for our transformation. Genotype: MATa, ade2-1, trp1-1, can1-100, leu2-3, 112, his3-11,15, ura3, GAL, psi+

4196

This strain is a derivate of the yeast strain BY4742, which carries an intact copy of the canamycin-gene which can be disrupted by homologous recombination. This was planned to be a second locus of interest next to trp. Genotype: BY4742; MATalpha; his3Δ1, leu2&Delta0, ura3&Delta0