Difference between revisions of "Team:HUST-China/Part Collection"

Best Part Collection:

Particularly,we did well in the standardization of E.coli 50S ribosomal protein (Si-tag) with the different combination of domains:1,2,3,1+2,1+3,2+3,1+2+3,1L3.

We added a signal peptide and cell wall protein respectively at N terminal and C terminal of the Si-tags which can immobilize the working protein on the cell wall to promote the protein’s function.

The results of fluorescence immunoassay showed that we succeeded in displaying the silica-tag onto the cell surface. Silica surface binding test shows each protein domain had different binding ability, so that we can choose different combinations of Si-tag domains to satisfy different requirement of binding intensity.