Difference between revisions of "Team:Tokyo Tech/Description"
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<h2 id="Summary" class="smalltitle">0. Summary</h2> | <h2 id="Summary" class="smalltitle">0. Summary</h2> | ||
<p class="text">iGEM 2015 Tokyo_Tech improved the function of <a href="http://parts.igem.org/Part:BBa_K395160:Experience">BBa_K395160</a> by submission of <a href="http://parts.igem.org/Part:BBa_K1632020">BBa_K1632020</a>, and characterization of <a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a>, to meet gold medal criteria.</p> | <p class="text">iGEM 2015 Tokyo_Tech improved the function of <a href="http://parts.igem.org/Part:BBa_K395160:Experience">BBa_K395160</a> by submission of <a href="http://parts.igem.org/Part:BBa_K1632020">BBa_K1632020</a>, and characterization of <a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a>, to meet gold medal criteria.</p> | ||
| − | <p class="text">Furthermore, we have improved characterization of another previously existing part, RNA thermometer (FourU) coding sequence plasmid (<a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a>), constructed by iGEM 2014 SYSU-China by the following three points. <b>(1)</b> We measured the function of <a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a> at 42 ºC, which wasn’t confirmed by iGEM 2014 SYSU-China. <b>(2)</b> We clarified the processing of the background derived from Negative control. <b>(3)</b> We explicated that the measuring equipment was flow cytometer. | + | <p class="text">Furthermore, we have improved characterization of another previously existing part, RNA thermometer (FourU) coding sequence plasmid (<a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a>), constructed by iGEM 2014 SYSU-China by the following three points. <b>(1)</b> We measured the function of <a href="http://parts.igem.org/Part:BBa_K1333309">BBa_K1333309</a> at 42 ºC, which wasn’t confirmed by iGEM 2014 SYSU-China. <b>(2)</b> We clarified the processing of the background derived from Negative control. <b>(3)</b> We explicated that the measuring equipment was flow cytometer. <br> |
| + | go to <h4 id="RNA">RNA thermometer</h4>.</p> | ||
<h2 id="ssrA" class="smalltitle">1. ssrAのパート</h2> | <h2 id="ssrA" class="smalltitle">1. ssrAのパート</h2> | ||
<h2 id="RNA" class="smalltitle">2. RNA thermometer</h2> | <h2 id="RNA" class="smalltitle">2. RNA thermometer</h2> | ||
| + | <h3 id="21" class="sub5">2.1. </h3> | ||
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</div> | </div> | ||
</body> | </body> | ||
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Revision as of 01:07, 18 September 2015
Description
contents
0. Summary
1. sstAのパート
2. RNA thermometer
2.1.“Background”for our characterization
2.2. Summary of the experiment
2.3. Result
3. Results
3.1. Arabinose-dependent FimB (wild-type) expression
3.2. FLA analysis
4. Discussion
5. Materials and Methods
5.1. Construction
5.2. Assay Protocol
5.2.1 Arabinose dependent FimB expression
5.2.2. FLA analysis
6. Reference
0. Summary
iGEM 2015 Tokyo_Tech improved the function of BBa_K395160 by submission of BBa_K1632020, and characterization of BBa_K1333309, to meet gold medal criteria.
Furthermore, we have improved characterization of another previously existing part, RNA thermometer (FourU) coding sequence plasmid (BBa_K1333309), constructed by iGEM 2014 SYSU-China by the following three points. (1) We measured the function of BBa_K1333309 at 42 ºC, which wasn’t confirmed by iGEM 2014 SYSU-China. (2) We clarified the processing of the background derived from Negative control. (3) We explicated that the measuring equipment was flow cytometer.
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