Difference between revisions of "Team:CU Boulder/Parts/team"

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<h2><b>BBa_K1718001:</b>   RFP under arabinose inducible pBad</h2>
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<h2>BBa_K1718001:</h2>
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<b>RFP under arabinose inducible pBad</b>
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<p>This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.</p>
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<h2>BBa_K1718002:</h2>
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<div id="section_02">
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<b>Genetic Switch for Bxb1 invertase</b>
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<p>The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.</p>
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<h2>BBa_K1718003:</h3>
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<div id="section_03">
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<b>RFP under the LuxPr promoter</b>
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<p>In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.</p>
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<h2>BBa_K1718004:</h2>
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<h2>BBa_K1718005:</h2>
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Revision as of 01:56, 18 September 2015


<!DOCTYPE html> Team:CU_Boulder - 2015.igem.org

Team Parts



BBa_K1718001:

RFP under arabinose inducible pBad

This part has a Red Fluorescent Protein (RFP) downstream of the pBad promoter. Expression is stimulated by L-arabinose. We used this part to test the leakiness of this promoter.

BBa_K1718002:

Genetic Switch for Bxb1 invertase

The Bxb1 invertase will invert the segment of DNA between the two recognition sites. In this case, Bxb1 will invert a terminator allowing for expression of a strong Anderson promoter.

BBa_K1718003:

RFP under the LuxPr promoter

In the presence of AHL, the LuxR transcription factor will bind AHL and activate the LuxPr promoter. RFP is downstream and will be expressed.

BBa_K1718004:

BBa_K1718005:

Team:CU-Boulder - 2015.igem.org