Difference between revisions of "Team:UFSCar-Brasil"

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  <p>Diseases transmitted by insect vectors, such as malaria, dengue fever, yellow fever and leishmaniasis, affect millions of people and caused about 600.000 deaths around the world in 2012, especially in tropical regions(1). In Brazil, population is affected
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    <h3 class="ui header" id="overview">Bug Shoo - an innovator repellent</h3>
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    significantly by those diseases. Considering this problem, our project consisted in developing an alternative repellent of that currently sold in the market, more or equally effective in preventing mosquitoes bites that transmits diseases. The main
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    <p>The Bug shoo is the representation of our project for society.</p>
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    compound in current repellents is
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<p>It is the way that our team designed a product, manufactured with the tools of synthetic biology, which may be effective in combating diseases transmitted by insect vectors, such as dengue. Its active ingredient is d-limonene, a gift terpene in cítrricas fruits and repellent action. Its production will be performed by plasmolisadas and bacteria that have been genetically programmed for that perform this function.</p>
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    <b>DEET (N, N-diethyl-m-toluamide)</b>, a toxic molecule which at certain concentrations could be lethal, and therefore must have strict control on its use (9). The main characteristic of our repellent is a longer duration when compared to other products
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     and the replacement of DEET by <b>D-limonene</b>, a less toxic compound. Our proposal was building a bacteria carrying out the production of D-limonene via limonene synthase. To enable long term storage at room temperatures, the bacterial cells that
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<p>Find out in our project the three main characteristics that make a single Shoo Bug repellent and could be presented later to the market. Also, follow along as our team has developed Policy & Practices of structured activities to raise awareness about dengue.</p>
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    make up the repellent would be plasmolyzed, with suspended metabolism, in a dormant state. The maintenance of this state would be obtained by a solution of polyethylene glycol (PEG) that will raise the osmotic pressure. Once in contact with the skin,
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     PEG solution would be diluted by sweat and inducing osmotic shock in bacteria cells. Then the universal stress protein promoter (UspA) would be activated, inducing expression of limonene synthase. In addition to PEG, other compounds such as glycerol
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    and metal ions in low concentrations would constitute our insect repellent cream, in order to keep bacteria at the required metabolic condition and posterior enzyme activities. This way, the distribution of our repellent may become feasible, allowing
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Revision as of 02:10, 18 September 2015

Home
Project
Overview
Issue Plasmolysis and Limonene synthase Protein Solubilization Toolkit Kill Switch
Team
Our Team Attributions
Achievements
Overview
Parts Results Judging Criteria
Policy & Practices
Overview
Political Social Educational Safety/Security Meetings Fundraising
Modeling
Overview
Plasmolysis Protein Solubilization Toolkit Kill Switch
Notebook
Protocols Problems Collaborations Safety Acknowledgements

Team UFSCar-Brasil

People helping people

Bug Shoo - an innovator repellent

The Bug shoo is the representation of our project for society.

It is the way that our team designed a product, manufactured with the tools of synthetic biology, which may be effective in combating diseases transmitted by insect vectors, such as dengue. Its active ingredient is d-limonene, a gift terpene in cítrricas fruits and repellent action. Its production will be performed by plasmolisadas and bacteria that have been genetically programmed for that perform this function.

Find out in our project the three main characteristics that make a single Shoo Bug repellent and could be presented later to the market. Also, follow along as our team has developed Policy & Practices of structured activities to raise awareness about dengue.

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