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| | <tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br> | | <tbody><tr><td style="padding: 5px 0px; width: 750px; font-size: 10pt; font-weight: bold;" colspan="9">Circular mRNA - the world's longest protein<br> |
Revision as of 03:33, 18 September 2015
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TEAM PROFILE
| Team Name: | Gifu | | Primary Contact (PI): | Hitoshi IWAHASHI | | Schools: | Gifu University
Yanagido, Gifu, Japan | | Division: | iGEM | Application Date: 2015-04-27 | | Section: | Undergraduate | | Region: | Asia | Acceptance Date: 2015-05-02 08:32:07 | | Description: |
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Welcome to iGEM 2015 ! Your iGEM 2015 team application was accepted by iGEM Headquarters on 2015-05-02 08:32:07
and your team registration fee has been received. |
| Assigned Track: New Application
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Circular mRNA - the world's longest protein
| | Generally, mRNA is single-strand RNA, starting translation by binding ribosome on initiation codon, and ending by separating ribosome from mRNA. In this study, we aim to build the method of synthesizing long-repeating proteins, massive one, and to improve translation efficiency. That is, allowing ribosomes to have a semi-permanent translation mechanism by producing circular mRNA and causing a defect of termination codon. To cyclize mRNA, we can use a splicing mechanism of T4 phage. Splicing is a mechanism removing circular introns and joining in exons after transcription. Splicing is catalyzed by several base sequences at both ends of intron as a ribozyme, being subjected to nucleophilic attack from introns to exons. We would like to clone the two parts of intron to use this mechanism and cyclize mRNA, making plasmids which include a gene coding proteins between those. And, by transforming E.coli, we get world’s longest proteins.
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| This range of part numbers has been assigned to your team for use during the summer: BBa_K1859000 to BBa_K1859999
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