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<h1> COLLABORATIONS </h1>
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<h1> HUMAN PRACTICE </h1>
  
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<h2> <b> Surveying the Land </b> </h2>
<p> One of the core value and spirit of iGEM is working together with other teams to solve problems and share resources. As such, we did our best to extend a helping hand when needed.</p>
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<h2> <b> Team UGA-Georgia (University of Georgia) </b> </h2>
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<p> The team wanted to get a preview of how our idea would be perceived by the general public. As a result, in order to get a better understanding of what the general public knows about GMOs and probiotics, we created an online survey. The goal was to have a survey that gradually gives more information about GMOs to measure initial comfort with GMOs, educate a wide range of people about what GMOs actually are, and measure their williness to support GMOs afterwards. The people who took the survey ranged from 18 to 50+ years old and had an education background ranging from high school to advanced profession degrees. The survey can be found <a href = "https://docs.google.com/a/columbia.edu/forms/d/1LRt45MPoeKxt540zvHK4xbwtq_E3vLZal4C_tVbCPPg/viewform"> here.</a> Please feel free to continue to contribute to it and expand our dataset! In addition, we acknowledge the fact that our sample size may be skewed towards people who are already interested in synthetic biology based on how we advertised it (through the team and personal forms of social media), but the information gleaned from the data we collected is still rather useful and allows us to be more insightful. </p>  
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<p> <a href = "http://www.uga.edu/"> <img src= "https://static.igem.org/mediawiki/2015/a/a3/Columbia_NYC_UGALogo.jpg" alt = "UGA Logo" align = "right" style = "width: 110px; margin-left: 20px;"> </a>
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<a href = "https://2015.igem.org/Team:UGA-Georgia"> UGA iGEM </a> has asked us to help in their effort to characterize archael ribosome binding sites by measuring the expression of mCherry in their sample as a proxy for RBS activity. As such, we measured the fluorescence and protein concentration of 30 samples (triplets of 10) at an excitation wavelength of 590nm and emission of 645nm and at OD280, respectively. </p>
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<img src = "https://static.igem.org/mediawiki/2015/2/2c/IMAG0249.jpg" alt = "Hudson UGA Help" style = "width: 1000px;">
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<img src = "https://static.igem.org/mediawiki/2015/2/23/Columbia_NYC_SurveyInitial.png" alt = "Survey Initial" align = "right" style = "width: 150px;">  
Hudson setting up the plate read for the 30 samples that UGA sent us
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<h2> <b> Team Genspace </b> </h2>
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<p> So, what exactly did we find out from our survey? Well for one, on a scale from 1-5 with 5 being the most comfortable, 58.3% of people has a comfort level less than or equal to 3. This confirms our suspicion that most people are not all too comfortable with the ideas of GMO.
<p> <a href = "http://genspace.org/"> <img src = "https://static.igem.org/mediawiki/2015/2/23/Columbia_NYC_GenspaceLogo.png" alt = "Genspace Logo" align = "left" style = "width: 350px; margin-right: 20px;"> </a>
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<p> <a href = "https://2015.igem.org/Team:Genspace"> Genspace iGEM </a> members were having trouble with a quorum-sensing related promoter that was controlling the expression of red fluorescent protein (RFP). The RFP was meant to only be expressed when a threshold cell density was met, but was being expressed below this threshold. As a result, we suggested that they had their construct's base RFP expression level measured via our fluorometer and how this compared to fluoresence levels after the threshold density was met to better characterize what was going on with their part. Since Genspace is a community biolab that is not as well-equiped as an institutional laboratory such as ours, we offered help by means of equipment that we are more fortunate to have. </p> 
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<img src = "https://static.igem.org/mediawiki/2015/4/45/Columbia_NYC_JackyHelpGenspace.jpg" alt = "Jacky Genspace Help" style = "width: 1000px;">
 
Jacky helping the Genspace Team measure their fluorescence
 
  
 
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Revision as of 04:15, 18 September 2015

HUMAN PRACTICE

Surveying the Land

The team wanted to get a preview of how our idea would be perceived by the general public. As a result, in order to get a better understanding of what the general public knows about GMOs and probiotics, we created an online survey. The goal was to have a survey that gradually gives more information about GMOs to measure initial comfort with GMOs, educate a wide range of people about what GMOs actually are, and measure their williness to support GMOs afterwards. The people who took the survey ranged from 18 to 50+ years old and had an education background ranging from high school to advanced profession degrees. The survey can be found here. Please feel free to continue to contribute to it and expand our dataset! In addition, we acknowledge the fact that our sample size may be skewed towards people who are already interested in synthetic biology based on how we advertised it (through the team and personal forms of social media), but the information gleaned from the data we collected is still rather useful and allows us to be more insightful.

Survey Initial

So, what exactly did we find out from our survey? Well for one, on a scale from 1-5 with 5 being the most comfortable, 58.3% of people has a comfort level less than or equal to 3. This confirms our suspicion that most people are not all too comfortable with the ideas of GMO.