Difference between revisions of "Team:SZU China/Practices"

Line 238: Line 238:
 
<div class="cbp_tmlabel">
 
<div class="cbp_tmlabel">
 
 
−
<img src="https://static.igem.org/mediawiki/2015/f/fe/SZU_China_WHJ_4.jpg">
+
<img src="https://2015.igem.org/File:SZU_China_WHJ_1.jpg">
−
                        <img src="https://static.igem.org/mediawiki/2015/d/de/SZU_China_WHJ_13.jpg">
+
−
                        <img src="https://static.igem.org/mediawiki/2015/6/61/SZU_China_WHJ_6.jpg">
+
−
                        <img src="https://static.igem.org/mediawiki/2015/f/f4/SZU_China_WHj_5.jpg">
+
 
</div>
 
</div>
 
</li>
 
</li>

Revision as of 09:52, 18 September 2015



Human practice




  • 1.The Festival exhibit of College of Life Sciences SZU
    Biological graduates are once considered to be one of the groups with lowest employment rate and biology has since been left out in many students’ mind. However, synthetic biology open a window for the university student to look deeper into the fantastic aspect that various biological parts can be synthesized like a computer. The Festival exhibit the last year and this year project of our team attached with the synthetic biological information. Surprisingly it receive some praise. Some literal students even the exchange students grew strong interest in our project and would like to join us in the lab.

  • Transfected the two plasmid system in 5637 and T24 bladder cancer cells,and got a good result in Rluc(with amber mutation). Moreover,we got the control experiments data in human bladder fiber epithelial normal cells(HFC).

  • Improved the original BioBrick TERT (BBa_K1722001).

  • In order to increase the expression of the system,we remoulded the system in three palsmid.

  • We greeted Tito Jankowski visiting SZU-iGEM,who is the world's first bio hackerspaces BioCurious founder.

  • Began to distribute questionnaires Onlinehttp://www.sojump.com/jq/5569949.aspx

  • Transfected the three plasmid system in the T24 and 5637 bladder cancer cells,but its result was not so good.Therefore,we continued to use the two plasmid system.

  • Propaganda synthetic biology and iGEM to all students at the Cultural Festival of the Collage of Life Science.

  • Finished some BioBricks:shTERT+GFP Composite(BBa_K1722009), TERT part(BBa_K1722002), shTERT part(BBa_K1722001),hUPII part(BBa_K1722000) and Rlu part(BBa_K1722005).

  • Added GFP(with amber mutation) to the two plasmid system, and transfected the system in 5637 and T24 bladder cancer cells.

  • Finished some BioBricks:SV40(en) part(BBa_K1722006), SV40+Rlu Composite(BBa_K1722013), tRNA part(BBa_K1722004), SV40(en)+Rlu Composite(BBa_K1722012)

  • LZU-iGEM and SZU-iGEM made a whole day of cooperative communication.

  • The GFP gene transfection test was successful, and it proved that THE BLADDER CANCER SNIPER SYSTERM was functional.

  • Finished the rest of BioBricks:ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite（BBa_K1722010），AckRS part(BBa_K1722003),hUPll+AckRS Composite(BBa_K1722007)

  • Finished the rest of BioBricks:hUPll+AckRS Composite(BBa_K1722007),AckRS part(BBa_K1722003),ShTERT+tRNA Composite(BBa_K1722011),hTERT+tRNA Composite(BBa_K1722010)

  • Finished collecting BioBricks data, and edited in the wiki.https://2015.igem.org/Team:SZU_China/Parts

  • we visited the Shenzhen Cancer Rehabilitation Association,and made a thorough understanding about specific situation of cancer patients.

  • In order to prove the system's function and specificity, we made second GFP transfection test.The result indicated the system has a good function and specificity.

  • According to our experience, we convened cancer patients,and made a cancer patients communication,Which purpose is to give patients confidence and improve the difficult situation of group's treatment in china.