Difference between revisions of "Team:Lethbridge HS/Results"

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Revision as of 11:02, 18 September 2015

Results

What results did we get in the lab?

Results

Oxalic acid results:

Oxalic Acid Concentration (mg/mL) OD528
0 1.7104
0.01 1.817 (only one trial)
0.5 1.647 (only one trial)
0.1 1.145125
0.15 1.6255 (only two trials)
0.2 0.7021428571
0.25 1.5195 (only two trials)
0.3 0.588375
0.35 1.696 (only one trial)
0.4 0.7051
0.45 1.737 (only one trial)
0.5 0.6271428571
0.55 1.666 (only one trial)
0.6 0.6502222222
0.65 1.674 (only one trial)
0.7 0.6052857143
0.75 1.597 (only one trial)
0.8 0.7459
0.85 1.533 (only one trial)
0.9 0.504
1.0 0.6849
1.4 0.789 (only two trials)
1.5 0.983 (only one trial)
1.6 0.8195 (only two trial)
1.8 0.047 (only one trials)
1.6 0.037 (only two trials)

Bee trials:

Treatment Before After
None Somewhat lethargic. Drank when prompted. Appeared tired, but otherwise unaffected.
0.00% Most lethargic, did not eat when offered Wet and slow moving.
0.05% Very vigorous; ran around inside the tube. Fed. Very energetic. Unaffected by treatment.
0.2% Very vigorous; ran around inside the tube. Fed. Slightly less energetic than the 0.05% bee.

The bees will be able to survive all of the oxalic acid that the E.coli can produce.

Mite trials:

Control Oxalic Acid RFP
Parafilm Pouch Mites dead Mites dead Mites dead
Larvae 1 Mites dead Mites dead Mites dead
Larvae 2 Mites dead Mites dead Mites dead

The mites did not survive any of the trials.

Antibiotics testing:

Test (Antibiotics) Result After
AMP 1 Positive
AMP 2 Positive Wet and slow moving.
CAM 1 Positive
CAM 2 Positive
TET 1 Positive
TET 2 Positive
Control (water) Positive

The results we got for antibiotics testing show that biofilms can still be recultured even after being treated with antibiotics.